Study on effects of space radiation using mouse embryonic stem cells kept long time in International Space Station

• Project/Area Number: 15K11919
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命科学
• Research Institution: Osaka City University
• Principal Investigator: Morita Takashi 大阪市立大学, 大学院医学研究科, 特任教授 (70150349)
• Co-Investigator(Kenkyū-buntansha): 吉田 佳世 大阪市立大学, 大学院医学研究科, 准教授 (30311921) ; 木津 あかね 大阪市立大学, 大学院医学研究科, 登録医 (30623201)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 宇宙放射線 / マウスES細胞 / 染色体異常 / DNA 損傷 / 国際宇宙ステーション / マウスＥＳ細胞 / DNA損傷 / 宇宙実験 / 胚性幹細胞

Outline of Final Research Achievements

It is important to evaluate the influence of space radiation on human body or mammalian cells during a longer stay in space including missions to International Space Station (ISS), the moon of the earth, or Mars. Mouse embryonic stem (ES) cells are good candidate to estimate the space radiation effects, because they have normal karyotypes of chromosomes.The frozen mouse ES cells were launched by Space X-II in March 1, 2013 as project named “Stem Cells”. The frozen ES cells have been kept in the Japanese experiment module called “KIBO” in ISS and stored in a freezer (MELFI) at -95C. The ES cells exposed to space radiation were examined by their DNA damages by detecting histone H2AX foci and their chromosomal aberrations by FISH. The ES cells were also microinjected into normal embryos and cultured in vitro to see the developmental defects. The ES cells were thawed and their viabilities, formation of chimeras and chromosome aberrations were tested.

Research Products

• [Int'l Joint Research] Prairie View A&M University(米国)
• [Int'l Joint Research] University of Nevada, Las Vega(米国)
• [Journal Article] Galactic cosmic ray simulation at the NASA Space Radiation Laboratory, (2016)
  • Author(s): (1)JW.Norbury T. Morita, et al
  • Journal Title: Life Sci Space Res Volume: 8 Pages: 38-51
  • DOI: 10.1016/j.lssr.2016.02.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gene-modified embryonic stem cell test to characterize chemical risks. (2015)
  • Author(s): Kitada K, Kizu A, Teramura T, Takehara T, Hayashi M, Tachibana D, Wanibuchi H, Fukushima S, Koyama M, Yoshida K, Morita T.
  • Journal Title: Environ Sci Pollut Res Int. Volume: 22 Issue: 22 Pages: 18252-18259
  • DOI: 10.1007/s11356-015-5051-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 国際宇宙ステーションで52か月間凍結保存したマウスES細胞への宇宙放射線影響の解析; シンポジウム「宇宙における放射線防護を考える-影響の多面性と評価-」 (2017)
  • Author(s): 吉田佳世，木津あかね，秦 恵，江口－笠井清美，寺村岳士，山崎千秋、鈴木ひろみ，嶋津 徹，永松愛子，鈴木智美、東端晃、矢野幸子，白川正輝，P. Saganti，笠原春夫 ，F. A. Cucinotta ，森田 隆
  • Organizer: 日本放射線影響学会第60回大会
  • Invited
• [Presentation] 宇宙放射線によるマウス凍結 ES 細胞への影響 (2017)
  • Author(s): 吉田佳世，木津あかね，秦 恵，江口－笠井清美，寺村岳士，山崎千秋、鈴木ひろみ，嶋津 徹5)，永松愛子，鈴木智美、東端晃、矢野幸子，白川正輝，P. Saganti，笠原春夫7，F. A. Cucinotta ，森田 隆
  • Organizer: 宇宙生物科学会第2回大会
• [Presentation] pace Experiment to estimate effects of space radiation mouse ES cells in ISS (2016)
  • Author(s): K. Yoshida, A. Kizu, K. Kitada, M. Hada2, K., Eguchi-Kasai, T. Teramura, H. Suzuki, T. Shimazu, S S. Yano, M. Shirakawa, I. Osada, H. Kasahara, F. A. Cucinotta, T. Morita.
  • Organizer: ワークショップ「粒子線を利用した研究へのいざない」日本放射線影響学会
  • Place of Presentation: ＪＭＳアステールプラザ（広島県広島市）
  • Year and Date: 2016-10-26
  • Invited
• [Presentation] 凍結マウスＥＳ細胞を用いた宇宙放射線影響の解析 (2016)
  • Author(s): 吉田佳世，木津あかね，北田紘平，秦 恵，江口－笠井清美，寺村岳士，鈴木ひろみ，嶋津 徹，永松愛子，矢野幸子，白川正輝，長田郁子 ，笠原春夫 ，F. A. Cucinotta ，森田 隆
  • Organizer: 宇宙航空環境医学会・宇宙生物科学会合同大会シンポジウム
  • Place of Presentation: 愛知医科大学たちばなホール（愛知県名古屋市）
  • Year and Date: 2016-10-13
  • Invited
• [Presentation] The Space Experiment Project (Stem Cells)) in International Space Station to Evaluate Effects of Space Radiation Using Mouse ES Cells, (2015)
  • Author(s): ①Yoshida K, Kizu A, Kitada K, Hada M, Eguchi-Kasai K, Teramura T. Suzuki, H.Shimazu, Yano, S, Shirakawa M, Osada, I, KasaharaH. Cucinotta FA, Morita T
  • Organizer: 15th Internation Congress of Radiation
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Presentation] Evaluation of Space Radiation Effects to Mouse Embryonic Stem (ES) Cells Stocked in MELFI in ISS for Fouirteen Months as “STEM CELLS”Project, (2015)
  • Author(s): ②Yoshida K, Kizu A, Kitada K, Hada M, Eguchi-Kasai K, Teramura T. Suzuki, H.Shimazu Nagamatsu A, Yano, S, Shirakawa M, Osada, I, KasaharaH. Cucinotta FA, Morita T
  • Organizer: Space Radiation and Heavy Ions in Therapy Symposium 2015
  • Place of Presentation: 阿倍野ハルカス会議室（大阪府大阪市）
  • Year and Date: 2015-05-22
  • Int'l Joint Research