Analysis of Cs uptake mechanism in Oryza sativa by using chemical mutants.

• Project/Area Number: 15K11956
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 復興農学
• Research Institution: Akita Prefectural University
• Principal Investigator: RAI HIROKI 秋田県立大学, 生物資源科学部, 助教 (30503099)
• Co-Investigator(Kenkyū-buntansha): 永澤 奈美子 (佐藤奈美子) 秋田県立大学, 生物資源科学部, 准教授 (00535289) ; 永澤 信洋 秋田県立大学, 生物資源科学部, 准教授 (90599268) ; 高橋 秀和 秋田県立大学, 生物資源科学部, 准教授 (00325937)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: セシウム / イネ / 低吸収 / カリウム輸送体 / OsHAK1 / 放射性核種 / 137Cs / 水稲 / 吸収抑制 / カリウム / 輸送体 / 福島第一原発 / 放射性セシウム / カリウムトランスポーター / HAK / 原発事故 / 福島 / 放射性 / セシウム低吸収 / 吸収制御遺伝子

Outline of Final Research Achievements

Nuclear incident at the Fukushima in 2011 have resulted in widespread soil contamination by caesium-137 which raised serious food security issues. We isolated rice mutants with low caesium uptake and reveal that the Oryza sativa potassium transporter OsHAK1, which is expressed on the surfaces of roots, is the main route of caesium influx into rice plants, especially in low-potassium conditions. During hydroponic cultivation with low-to-normal potassium concentrations, caesium influx in oshak1 was no greater than one-eighth that in wild type. In field experiments, oshak1 showed dramatically reduced caesium concentrations in grains and shoots, but their potassium uptake was not greatly affected and their grain yields were similar to that of wild type. These results show that low-caesium-uptake rice lines can be developed for cultivation in radiocaesium-contaminated areas.

Research Products

• [Journal Article] Cesium Uptake by Rice Roots Largely Depends Upon a Single Gene, HAK1, Which Encodes a Potassium Transporter. (2017)
  • Author(s): Hiroki Rai, Saki Yokoyama, Namiko Satoh-Nagasawa, Jun Furukawa, Takiko Nomi, Yasuka Ito, Shigeto Fujimura, Hidekazu Takahashi, Ryuichiro Suzuki, ELMannai Yousra, Akitoshi Goto, Shinichi Fuji, Shin-ichi Nakamura, Takuro Shinano, Nobuhiro Nagasawa, Hiroetsu Wabiko, Hiroyuki Hattori.
  • Journal Title: Plant and cell physiology Volume: 58 Issue: 9 Pages: 1468-1493
  • DOI: 10.1093/pcp/pcx094
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The high correlation between DNA and chloroform-labile N in various types of soil. (2017)
  • Author(s): Yokoyama,S., Yuri,K., Nomi,T., Komine,M., Nakamura,S., Hattori,H. and Rai,H.
  • Journal Title: Applied Soil Ecology Volume: 117-118 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Genetic analysis of cadmium accumulation in shoots of sorghum landraces. (2017)
  • Author(s): Tsuboi,K., Shehzad,T., Yoneda,J., Uraguchi,S., Ito,Y., Shinsei,L., Morita,S., Rai,H., Nagasawa,N., Asari,K., Suzuki,H., Itoh,R., Saito,T., Suzuki,K., Takano,I., Takahashi,H., Sakurai,K., Watanabe,A., Akagi,H., Tokunaga,T., Itoh,M., Hattori,H., Fujiwara,T., Okuno,K., Tsutsumi,N. and Satoh-Nagasawa,N.
  • Journal Title: Crop Science Volume: 57(1) Pages: 22-31
  • Peer Reviewed
• [Presentation] セシウム低吸水稲のCs/K吸収特性と現地試験結果 (2017)
  • Author(s): 頼 泰樹、横山 咲、佐藤奈美子、古川 純、能美多希子、伊藤那香、森田祥司、藤村恵人、後藤明俊、信濃卓郎、永澤信洋、我彦廣悦、中村進一、服部浩之
  • Organizer: 日本土壌肥料学会
• [Presentation] セシウム低吸収の水稲突然変異体の吸収制御遺伝子解析 (2016)
  • Author(s): 頼泰樹、横山咲、由利かほる、佐藤奈美子、永澤信洋、高橋秀和、藤晋一、保田謙太郎、中村進一、服部浩之
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 佐賀大学
  • Year and Date: 2016-09-20
• [Presentation] セシウムを吸わない突然変異水稲の開発とその解析 (2015)
  • Author(s): 頼 泰樹、横山 咲、由利 かおる、佐藤 奈美子、永澤 信洋、高橋 秀和、藤 晋一、中村 進一、服部 浩之
  • Organizer: 日本土壌肥料学会 2015年度京都大会
  • Place of Presentation: 京都大学
  • Year and Date: 2015-09-09
• [Presentation] Development of low caesium uptake oryza sativa by the chemical mutangen. (2015)
  • Author(s): H. Rai, S. Yokoyama, K. Yasuda, S. Fuji, N. Sato, N. Nagasawa, H. Takahashi, S. Nakamura, H. Hattori
  • Organizer: 13th International Conference on the biogeochemistry of trace elements (ICOBTE 2015)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] セシウム吸収を制御する遺伝子及びセシウム低吸収植物 (2016)
  • Inventor(s): 頼泰樹ら
  • Industrial Property Rights Holder: 公立大学法人 秋田県立大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-167064
  • Filing Date: 2016-08-29