| Project/Area Number |
15K12138
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Life / Health / Medical informatics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Sawai Satoshi 東京大学, 大学院総合文化研究科, 教授 (20500367)
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| Co-Investigator(Kenkyū-buntansha) |
片桐 晃子 北里大学, 理学部, 教授 (00322157)
木梨 達雄 関西医科大学, 医学部, 教授 (30202039)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 走化性 / 免疫細胞 / 数理モデル / 生命情報 / 好中球 / 細胞運動 / マイクロ流体デバイス / 細胞遊走 |
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| Outline of Final Research Achievements |
The project aimed to understand cellular sensory perception of migrating immune cells with an emphasis on spatio-temoral dynamics. Microfluidics gradient chamber was employed to generate a bell-shaped gradient, and by precise flow control, it was propagated in space at a specified speed. We found that neutrophil-like HL60 cells exhibit specific and assymetric migratory patterns depending on the spatio-temporal property of the imposed dynamic gradients . We analyzed spatio-temporal dynamics of the major signaling components relative to the observed migratory response by employing live cell time lapse imaging of cells expressing FRET-sensors and other fluorescent marker proteins. The obtained signaling response suggests close relation between the observed migratory behavior and the spatio-temporal property of the signaling activities.
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