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Analysis of spatio-temporal information processing in immune cells

Research Project

Project/Area Number 15K12138
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Life / Health / Medical informatics
Research InstitutionThe University of Tokyo

Principal Investigator

Sawai Satoshi  東京大学, 大学院総合文化研究科, 教授 (20500367)

Co-Investigator(Kenkyū-buntansha) 片桐 晃子  北里大学, 理学部, 教授 (00322157)
木梨 達雄  関西医科大学, 医学部, 教授 (30202039)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords走化性 / 免疫細胞 / 数理モデル / 生命情報 / 好中球 / 細胞運動 / マイクロ流体デバイス / 細胞遊走
Outline of Final Research Achievements

The project aimed to understand cellular sensory perception of migrating immune cells with an emphasis on spatio-temoral dynamics. Microfluidics gradient chamber was employed to generate a bell-shaped gradient, and by precise flow control, it was propagated in space at a specified speed. We found that neutrophil-like HL60 cells exhibit specific and assymetric migratory patterns depending on the spatio-temporal property of the imposed dynamic gradients . We analyzed spatio-temporal dynamics of the major signaling components relative to the observed migratory response by employing live cell time lapse imaging of cells expressing FRET-sensors and other fluorescent marker proteins. The obtained signaling response suggests close relation between the observed migratory behavior and the spatio-temporal property of the signaling activities.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (26 results)

All 2018 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 4 results,  Invited: 9 results) Book (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Fold-change detection and scale-invariance of cell-cell signaling in social amoeba2017

    • Author(s)
      Kamino, K, Kondo, Y., Nakajima, A., Honda-Kitahara M., Kaneko, K., Sawai S.
    • Journal Title

      Proc. Nat. Acad. Sci. USA

      Volume: 114 Issue: 21

    • DOI

      10.1073/pnas.1702181114

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Microfluidic lighthouse: an omnidirectional gradient generator2016

    • Author(s)
      Akihiko Nakajima, Motohiko Ishida, Taihei Fujimori, Yuichi Wakamoto, Satoshi Sawai
    • Journal Title

      Lab on Chip

      Volume: 16(22) Issue: 22 Pages: 4382-4394

    • DOI

      10.1039/c6lc00898d

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 変動する誘引物質濃度勾配への好中球様HL60細胞の追従性能の解析2018

    • Author(s)
      石田 元彦
    • Organizer
      新学術領域「数理シグナル」第2回公開シンポジウム 若手研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 動く細胞の情報処理特性の理解に向けて2018

    • Author(s)
      澤井 哲
    • Organizer
      自然科学研究機構新分野創成センターシンポジウム「分野横断・分野融合研究による生命創成を探究する新しい科学の創成」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 細胞性粘菌にみる微生物の集団性:振動、波、走化性2017

    • Author(s)
      澤井 哲
    • Organizer
      第4回ERATO学術セミナー
    • Place of Presentation
      筑波大学総合研究棟(茨城県つくば市)
    • Year and Date
      2017-02-06
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 時空間的に変化する誘引物質場における好中球様HL60細胞の走化性運動の解析(ポスター)2017

    • Author(s)
      石田元彦
    • Organizer
      定量生物の会第8回年会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2017-01-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Spatio-temporal constraints on cellular sensing: what it means for universal biology2017

    • Author(s)
      Satoshi Sawai
    • Organizer
      International Symposium of the origin of life ? synergy among the RNA: protein and lipid worlds
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 進行波刺激に対するHL60細胞の応答解析2017

    • Author(s)
      石田 元彦
    • Organizer
      新学術領域「数理シグナル」若手研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 動的な誘引場における好中球様HL60細胞の走化性解析2017

    • Author(s)
      石田 元彦
    • Organizer
      新学術領域「数理シグナル」第1回若手ワークショップ
    • Related Report
      2017 Annual Research Report
  • [Presentation] 走化性細胞遊走の時空間情報処理特性2017

    • Author(s)
      中島昭彦
    • Organizer
      2017年度応用数理学会 数理医学サブシンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Chemotactic analysis of neutrophil-like HL60 cells based on cells persistent polarity and immediate responsiveness to chemoattractant2017

    • Author(s)
      石田 元彦
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 走化性における適応応答依存的な時間情報コーディング2016

    • Author(s)
      澤井 哲
    • Organizer
      第39回日本分子生物学会年会シンポジウム「シグナル伝達における時間情報のコーディングシステム」
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] ライブセルイメージングと薬剤実験に基づく動的な誘引物質勾配場におけるHL60細胞の走化性運動の解析2016

    • Author(s)
      石田元彦 中島昭彦 澤井哲
    • Organizer
      生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-27
    • Related Report
      2016 Research-status Report
  • [Presentation] Microfluidic analysis of collective cell migration during contact-following in Dictyostelium2016

    • Author(s)
      Satoshi Sawai
    • Organizer
      STATPhys26
    • Place of Presentation
      Centre de Congres de Lyon(Lyon, France)
    • Year and Date
      2016-07-21
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Microfluidic analysis of persistence and reorientation of cell migration during contact-following in Dictyostelium2016

    • Author(s)
      澤井 哲
    • Organizer
      第68回日本細胞生物学会年会ミニシンポジウム
    • Place of Presentation
      京都テルサ(京都府京都市)
    • Year and Date
      2016-06-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Delineating temporal and spatial aspects of directional sensing in migrating cells2016

    • Author(s)
      中島 昭彦
    • Organizer
      4th Annual Winter Q-Bio Meeting
    • Place of Presentation
      Oahu - Sheraton Waikiki, Hawaii
    • Year and Date
      2016-02-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Revisiting cell migration mechanisms of crawling cells2016

    • Author(s)
      澤井 哲
    • Organizer
      定量生物学会 東京シンポジウム
    • Place of Presentation
      京大学駒場Ⅱキャンパス(東京都目黒区)
    • Year and Date
      2016-01-10
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Asymmetric chemotaxis response of neutrophil-like HL60 cells to temporally increasing/decreasing fMLP gradient stimulus2016

    • Author(s)
      石田 元彦
    • Organizer
      定量生物学会 東京シンポジウム
    • Place of Presentation
      東京大学駒場Ⅱキャンパス(東京都目黒区)
    • Year and Date
      2016-01-09
    • Related Report
      2015 Research-status Report
  • [Presentation] 動的なfMLP勾配刺激にたいする好中球様 HL60細胞の走化性応答2015

    • Author(s)
      石田 元彦
    • Organizer
      BMB2015(第38回日本分子生物学会年会及び第88回日本生化学大会合同大会)
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report
  • [Presentation] Dissecting temporal and spatial information for direction sensing in migrating cells2015

    • Author(s)
      中島 昭彦
    • Organizer
      ICSB2015 (16th International Conference on Systems Biology)
    • Place of Presentation
      Biopolis, Singapore
    • Year and Date
      2015-11-24
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 物理科学雑誌パリティ『特集 ゆらぎと構造からみる非平衡の世界』 Vol.32 (11)「細胞内反応場のゆらぎと細胞運動」2017

    • Author(s)
      石原秀至、澤井哲
    • Total Pages
      71
    • Publisher
      丸善出版
    • Related Report
      2017 Annual Research Report
  • [Book] 実験医学35(5)増刊号「時空間的なシグナルの検出とは何か ―這いまわる細胞の走化性を例に」2017

    • Author(s)
      澤井 哲
    • Total Pages
      5
    • Publisher
      羊土社
    • Related Report
      2016 Research-status Report
  • [Book] 生物物理56(2)「動く細胞が読み取る時間と空間:走化性のパラドクスと整流作用」(カバーイラスト)2016

    • Author(s)
      中島昭彦, 石原秀至, 澤井 哲
    • Total Pages
      4
    • Publisher
      日本生物物理学会
    • Related Report
      2016 Research-status Report
  • [Book] Chemotaxis: Methods and Protocols 2nd Ed. (Dale Hereld, Tian Jin編) Methods in Molecular Biology 1407, "Dissecting Spatial and Temporal Sensing in Dictyostelium Chemotaxis Using a Wave Gradient Generator" DOI: 10.1007/978-1-4939-3480-5_82016

    • Author(s)
      Nakajima, A., Sawai, S.
    • Total Pages
      16
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report
  • [Remarks] 生命システムの物理学 澤井研究室

    • URL

      http://sawailab.c.u-tokyo.ac.jp/

    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 流体流制御装置及び流体流制御方法2016

    • Inventor(s)
      澤井哲 中島昭彦
    • Industrial Property Rights Holder
      澤井哲 中島昭彦
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-04-26
    • Related Report
      2016 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2024-12-25  

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