Large-scale distribution fo virome along the thermohaline circulation

Research Project

Project/Area Number	15K12189
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Environmental dynamic analysis
Research Institution	Japan Agency for Marine-Earth Science and Technology
Principal Investigator	YOKOKAWA Taichi 国立研究開発法人海洋研究開発機構, 海洋生命理工学研究開発センター, 研究員 (00402751)
Co-Investigator(Renkei-kenkyūsha)	YAMADA Namiha 国立研究開発法人産業技術総合研究所, 環境管理技術研究部門, 主任研究員 (90435769) NAGATA Toshi 東京大学, 大気海洋研究所, 教授 (40183892)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	深海微生物生態 / ウイルス組成 / 海洋深層 / 海洋深層大循環 / 微生物生態系 / virome解析 / ウイルス叢
Outline of Final Research Achievements	We examined the distribution pattern of viral communities of deep-water samples along the “Thermohaline circulation (transit time: 1500 years; distance: 40000km)” in the Atlantic and the Pacific. We used a novel viromic approach, which is a combination of flowcytometer-sorting technique and whole genome amplification, to produce dsDNA-enriched viromic libraries from a 1-mL water sample. Using this approach on the environmental samples, we managed to produce nine libraries from the Atlantic samples and seven libraries form the Pacific samples. All the libraries composed of 12 broad taxonomic groups. Taxonomic compositions among the samples were different and changed dynamically along the thermohaline circulation. Our data demonstrate that viral taxonomic compositions are useful parameters for examining the dynamics of viral communities and the host-virus system in the ecosystem of the deep ocean realm.