Species differences and evolution of phase II conjugation reaction and its cellular function
| Project/Area Number |
15K12213
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
水川 葉月 北海道大学, 獣医学研究科, 助教 (60612661)
中山 翔太 北海道大学, 獣医学研究科, 助教 (90647629)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 異物代謝第II相抱合反応 / グルクロン酸 / UGT / 生物種差 / 細胞内デリバリー |
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| Outline of Final Research Achievements |
UGTs are among the most important xenobiotic metabolizing enzymes that conjugate a wide range of endogenous and exogenous compounds. Previous studies showed that Felidae species and Pinnipedia species have very low UGT activities toward some phenolic compounds because of the UGT1A6 pseudogene and small numbers of UGT1A isozymes. In addition to the UGT1As, UGT2Bs isozymes also conjugate various endogenous and exogenous compounds. Results of UGT activity measurement indicated cats and pinniped species had very low activities toward these substrates. Furthermore, Felidae species had the same nonsense mutation in UGT2B, which suggested that Felidae UGT2B31 was also a pseudogene in addition to UGT1A6. Together with the lower activity of UGT1As, these findings of lower activity of UGT2B suggested that Felidae and some pinniped species have very low UGT activity toward a wide range of chemicals.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Identification of inter-specific differences in phase II reactions: Determination of metabolites in the urine of 16 mammalian species exposed to environmental pyrene.2015
Author(s)
Saengtienchai A, Ikenaka Y, Nakayama SMM, Mizukawa H, Kakehi M, Bortey-Sam N, Darwish W, Tsubota T, Terasaki M, Poapolathep A, Ishizuka M
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Journal Title
Environmental Toxicology and Chemistry
Volume: 33
Issue: 9
Pages: 2062-2069
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 代謝酵素の動物種差2017
Author(s)
石塚真由美、中山翔太、水川葉月、池中良徳
Organizer
日本薬学会第137年会シンポジウム
Place of Presentation
仙台国際センター、仙台市
Year and Date
2017-03-24
Related Report
Invited
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