Construction of screening method of epi-mutagens based on control of intracellular DNase I
| Project/Area Number |
15K12214
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | University of Shizuoka |
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Principal Investigator |
Ibuki Yuko 静岡県立大学, 食品栄養科学部, 教授 (30236781)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | エピジェネティクス / DNase I / 界面活性剤 / ヒストン / クロマチン構造 / 熱 / γ-H2AX / 化学物質 |
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| Outline of Final Research Achievements |
We found that some kinds of surfactants like nonylphenol polyethoxylates and linear alkylbenzene sulfonates induced phosphorylation of histone H2AX (γ-H2AX ) via release of free deoxyribonuclease I (DNase I) with filamentous actin disruption. DNase I was translocated from the cytosol to the nucleus of cells after treatment with surfactants, and generated DNA strand breaks. The surfactant-induced generation of γ-H2AX increased relative to the acetylation caused by the treatment with histone deacetylase inhibitor, suggesting that the change of chromatin structure induced by environmental chemicals might be detectable by surfactant-induced γ-H2AX. On the other hand, we also found that heat stress induced similar release of DNase I and generation of γ-H2AX. This might be useful to establish the evaluation method for chromatin structure.
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Report
(3 results)
Research Products
(8 results)
