Research Project
Grant-in-Aid for Challenging Exploratory Research
Sick Building Syndrome(SBS) is caused by volatile organic compounds and organophosphorus(OP). However, despite the presence of SBS symptoms in patients the actual pathogenesis has not yet been developed.Since OP covalently bind Neuropathy Target Esterase (NTE) we construct the transgenic mice carrying human PNPLA6 cDNA which codes human NTE. The skin of the mice could digest the ester bond specific for human NTE and showed the atrophy of pyramidal cells in hippocampus and the Purkinje cells in cerebellum after exposure for high dose formalin. We also developed the new mutant mice of pnpla6 gene using CRISPR/ Cas9 system. Exposure of OP to chick embryos cause hemorrhage at the top of the brain. The NTE expression in a human brain died by drinking OP decreased and paralysis and aggregation of granular cells in the cerebellum occurred.Using human PNPLA6 cDNA, we made the part of NTE which contain active site in E.coli cells and human cells and partial purification was completed
All 2017 2016 2015
All Journal Article (3 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 3 results, Open Access: 2 results, Acknowledgement Compliant: 1 results) Presentation (8 results)
Biochem Biophys Res Commun
Volume: 491 Issue: 4 Pages: 1098-1104
10.1016/j.bbrc.2017.08.019
Japanese Journal of Clinical Ecology
Volume: 25 Pages: 49-54
臨床環境医学
Volume: 24 Pages: 88-93
