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Development of novel zero-GHG emission denitrifying process using denitrifying methane-oxidizing microorganisms.

Research Project

Project/Area Number 15K12225
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental engineering and reduction of environmental burden
Research InstitutionNagaoka University of Technology

Principal Investigator

Hatamoto Masashi  長岡技術科学大学, 産学融合トップランナー養成センター, 特任准教授 (20524185)

Co-Investigator(Kenkyū-buntansha) 山口 隆司  長岡技術科学大学, 工学研究科, 教授 (10280447)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsメタン / 脱窒 / 温室効果ガス / 亜酸化窒素 / 水処理 / 微生物 / アナモックス / メタン酸化 / アンモニア / 嫌気性メタン酸化 / 嫌気性アンモニア酸化
Outline of Final Research Achievements

n this study, anaerobic ammonium oxidation (anammox) and denitrifying anaerobic methane oxidization (DAMO) reaction were used for development of nitrogen removal process. Using the both microorganisms in continuous flor reactor or down - flow hanging sponge (DHS) reactor, both of reactor system could be removed the ammonia and nitrate simultaneously with methane as a carbon source. The consumed methane quantities were stoichiometrically close to the theoretical value in DAMO reaction suggested that DAMO reaction was occurred in our continuous culture. However, ammonia removal rate was lower than that of original annamox reactor, which is assumed that the nitrate reduction to nitrite by DAMO microorganism is rate-limiting step. In addition, nitrous oxide gas could be detected in the reactor. Thus, further research require more careful monitoring for relationship between green gas house emission and operational condition.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Cultivation of denitrifying anaerobic methane-oxidizing microorganisms in a continuous-flow sponge bioreactor2017

    • Author(s)
      Masashi Hatamoto, Takafumi Sato, Sho Nemoto, Takashi Yamaguchi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 印刷中 Issue: 14 Pages: 5881-5888

    • DOI

      10.1007/s00253-017-8315-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] アナモックス反応と嫌気的メタン酸化脱窒反応を組み合わせた窒素除去システムの性能評価2016

    • Author(s)
      吉田悠亮、根本笙、幡本将史、山口隆司
    • Organizer
      平成28年度土木学会全国大会
    • Place of Presentation
      仙台
    • Year and Date
      2016-09-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Application of denitrifying methane-oxidizing microorganisms for wastewater treatment using sponge bioreactor2016

    • Author(s)
      Masashi Hatamoto, Takafumi Sato, Shou Nemoto, Yusuke Yoshida, Takashi Yamaguchi
    • Organizer
      16th International Symposium on Microbial Ecology (ISME16)
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 嫌気的メタン酸化脱窒反応とアナモックス反応を組み合わせた窒素除去システムの検討と保持汚泥の微生物解析2016

    • Author(s)
      吉田悠亮, 根本笙, 幡本将史, 山口隆司
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      徳島
    • Year and Date
      2016-03-16
    • Related Report
      2015 Research-status Report
  • [Presentation] 嫌気的メタン酸化脱窒微生物の最適な培養条件の検討とDHSリアクターへの適用2016

    • Author(s)
      根本笙, 幡本将史, 山口隆司
    • Organizer
      第50回日本水環境学会年会
    • Place of Presentation
      徳島
    • Year and Date
      2016-03-16
    • Related Report
      2015 Research-status Report
  • [Presentation] アナモックス菌と嫌気的メタン酸化脱窒菌を利用した窒素除去システムの検討2015

    • Author(s)
      吉田悠亮, 根本笙, 幡本将史, 山口隆司
    • Organizer
      第33回 土木学会関東支部新潟会研究調査発表会
    • Place of Presentation
      新潟
    • Year and Date
      2015-11-13
    • Related Report
      2015 Research-status Report
  • [Presentation] 嫌気的メタン酸化脱窒微生物の集積培養と脱窒活性に与える基質組成の影響2015

    • Author(s)
      根本 笙, 佐藤 崇文, 幡本 将史, 山口 隆司
    • Organizer
      平成27年度土木学会全国大会 第70回年次学術講演会
    • Place of Presentation
      岡山
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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