| Project/Area Number |
15K12237
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
YOSHIDA NAOKO 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (10432220)
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| Co-Investigator(Kenkyū-buntansha) |
片山 新太 名古屋大学, 未来材料・システム研究所, 教授 (60185808)
押木 守 長岡工業高等専門学校, 環境都市工学科, 准教授 (90540865)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脱ハロゲン化呼吸 / バイオレメディエーション / ジオバクター / 脱ハロゲン化 / Geobacter / バイオオーグメンテーション / 脱塩素化 / ジクロロエタン |
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| Outline of Final Research Achievements |
We have isolated Geobacter sp. AY which detoxify 1,2-dichloroethane (1,2-DCA) to ethylene. In the kinetic analysis, strain AY showed the much higher dechlorination rate than yet-reported dehalorespiration reported so far. For exampl1E+8 cells/mL of cell density. In this study, a fed-batch cultivation using 10L-scale farmentor was performed to get more biomass of the strain AY for the application in bioaugmentation. As the result, 4.9 E+12 cells were obtained whithin 3-4 days from the initial cell density at 3E+9 cells/Ｌ. The kinetics of 1,2-DCA dechlorination by the strain was well fitted with Michaelis-Menten equation with irreversible substrate inhibition model. By using the parameters estimated based on the model, i.e. Ks, Kiu, Vmax, the changes of 1,2-DCA and ethylene in the fed-batch reactor were calculated and showed similar values with the actual experimental data. These results suggest the model and estimated parameters appropriately expressed the phenomenon in the reactor.
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