| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Development of a genetically engineered microorganism (GEM) able to grow on trichloroethene (TCE) was carried out for cleanup a TCE contaminated site aerobically. Phenol hydroxylase (PH) is known to degrade TCE completely via glyoxylate. We tried to develop a new GEM that degrade TCE efficiently. After analyzing TCE degradability of PH genes, a new hybrid PH gene made from 2 different PH genes was developed to make a GEM that could degrade TCE efficiently. Also a Cupriavidus bacterium was used as a host of the gene, because that bacterium was able to use glyoxylate as a sole carbon source for growth. However, we did not succeeded in decreasing toxicity of TCE epoxide by introducing etnE that metabolize vinyl chloride (VC) epoxide.
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