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Simple conversion of unused proteins in food wastes into new fluorescent materials

Research Project

Project/Area Number 15K12247
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental conscious materials and recycle
Research InstitutionNihon University

Principal Investigator

MATSUFUJI Hiroshi  日本大学, 生物資源科学部, 教授 (70287605)

Co-Investigator(Kenkyū-buntansha) 岩淵 範之  日本大学, 生物資源科学部, 准教授 (90328708)
Research Collaborator SAKANO Yuki  
HIROKAWA Yumi  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords食品廃棄物 / 再資源化 / 廃棄物利用 / 有機蛍光物質 / 未利用たんぱく質 / リグニン / 3-O-メチルガレート / リサイクル
Outline of Final Research Achievements

Reducing food losses and waste is an increasing global interest. We previously found that fluorescent substances were produced from 3-O-methylgallate (3-MGA) by a non-biological process. In this study, we examined a simple method for transforming proteins in food into fluorescent substances via a chemical reaction using 3-MGA. Fluorescent substances were produced effectively by incubation of 3-MGA and Tryptone at neutral pH at 28 C for 2 days. The antioxidants inhibited the production of fluorescent substances, but other food ingredients showed no inhibition. The incubation of 3-MGA with pancreatic hydrolysates of several proteins also produced fluorescent products. Finally, the incubation of 3-MGA with pancreatic hydrolysate of crude caseins prepared from commercial milk produced fluorescent substances. The simple transformation will contribute to the development of new basic techniques for the effective utilization of proteins in food losses and waste.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015 Other

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Int'l Joint Research: 1 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Chemical characterization and biological activity in young sesame leaves (Sesamum indicum L.) and changes in iridoid and polyphenol content at different growth stages.2018

    • Author(s)
      Y. Fuji, A. Uchida, K. Fukahori, M. Chino, T. Ohtsuki, H. Matsufuji
    • Journal Title

      PLoS ONE

      Volume: 13 Issue: 3 Pages: 1-15

    • DOI

      10.1371/journal.pone.0194449

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a Simple Nonbiological Method for Converting Lignin-Derived Aromatics into Nonaromatic Polymeric Substances with Fluorescent Activity.2016

    • Author(s)
      Noriyuki Iwabuchi, Yuki Sakano, Hajime Takiguchi, Hayato Takihara, Michio Sunairi, and Hiroshi Matsufuji.
    • Journal Title

      ACS Sustainable Chemistry & Engineering

      Volume: 4 Issue: 8 Pages: 4411-4416

    • DOI

      10.1021/acssuschemeng.6b01009

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Transformation of lignin-derived aromatics into non-aromatic polymeric substances with fluorescent activities (NAPSFA) by Pseudomonas sp. ITH-SA-12015

    • Author(s)
      N. Iwabuchi, H. Takiguchi, T. Hamaguchi, H. Takihara, M. Sunairi, H. Matsufuji
    • Journal Title

      ACS Sustainable Chemistry & Engineering

      Volume: 3 Issue: 11 Pages: 2678-2685

    • DOI

      10.1021/acssuschemeng.5b00503

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Purification and partial characterization of an X-prolyl-dipeptidyl aminopeptidase from Lactobacillus gasseri ME-2842015

    • Author(s)
      R. Miyabe, Y. Takahashi, H. Matsufuji, J. Ogihara, K. Itou, Y. Kawai, T. Masuda, K. Suzuki, M. Oda
    • Journal Title

      Bioscience, Biotechnology and Biochemistry

      Volume: 21 Pages: 445-451

    • NAID

      130005098074

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 市販牛乳酵素分解物からの有機蛍光物質生産について2018

    • Author(s)
      廣川侑美,大槻崇,岩淵範之,松藤寛
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] リグニン由来の芳香族化合物を変換して生産されるベンゼン環構造を含まない新規有機蛍光物質について2018

    • Author(s)
      岩淵範之,坂野優稀,坂本遼,松藤寛,砂入道夫
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Pseudomonas sp. ITH-B52の生産する有機蛍光物質の化学的性質の検討2018

    • Author(s)
      守屋佑里子,遠藤実夏,坂野優稀,松藤寛,岩淵範之,砂入道夫
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] タンパク質-パンクレアチン分解物と3-MGAの反応により生成する有機蛍光物質について2017

    • Author(s)
      廣川侑美,高野浩樹,関華奈子,坂野優稀,岩淵範之,大槻崇,松藤寛
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] アミンと3-MGAの反応により生成する有機蛍光物質について2017

    • Author(s)
      松藤寛,菅原一真,廣川侑美,坂野優稀,岩淵範之,大槻崇
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] ベンゼン環を含まない新規有機蛍光物質の非バイオプロセスによる生産2017

    • Author(s)
      坂野優稀,砂入道夫,松藤寛,岩淵範之
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 木質バイオマスからのベンゼン環を含まない新規有機蛍光物質の生産2017

    • Author(s)
      岩淵範之,坂野優稀,砂入道夫,松藤寛
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] たんぱく質-パンクレアチン分解物からの有機蛍光物質生産について2017

    • Author(s)
      廣川侑美,大槻崇,千野誠,岩淵範之,松藤寛
    • Organizer
      日本食品科学工学会平成29年度関東支部大会
    • Place of Presentation
      山梨学院大学(山梨県甲府市)
    • Year and Date
      2017-03-04
    • Related Report
      2016 Research-status Report
  • [Presentation] 食品廃棄物中未利用たんぱく質の有機蛍光物質への変換について2017

    • Author(s)
      廣川侑美,大槻崇,岩淵範之,松藤寛
    • Organizer
      日本食品科学工学会第64回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 食品廃棄物系バイオマスからのベンゼン環を含まない新規有機蛍光物質の生産2017

    • Author(s)
      廣川侑美,大槻崇,岩淵範之,松藤寛
    • Organizer
      日本食品化学学会第23回総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Transformation of lignin-derived aromatics into nonaromatic polymeric substances with fluorescent activities by Pseudomonas sp. ITH-SA-1.2016

    • Author(s)
      Y. Sakano, H. Matsufuji, M. Sunairi, N. Iwabuchi
    • Organizer
      The 11th SPSJ International Polymer Conference (IPC2016)
    • Place of Presentation
      Fukuoka International Congress Center(福岡県福岡市)
    • Year and Date
      2016-12-13
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 未利用たんぱく質の有機蛍光物質への簡易化学変換に関する研究2016

    • Author(s)
      廣川侑美,大槻崇,千野誠,坂野優稀,岩淵範之,松藤寛
    • Organizer
      日本食品科学工学会第63回大会
    • Place of Presentation
      名城大学(愛知県名古屋市)
    • Year and Date
      2016-08-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 低分子リグニン類から生産されるベンゼン環を含まない新規有機蛍光物質の生成機構の検討2016

    • Author(s)
      坂野優稀、松藤寛、砂入道夫、岩淵範之
    • Organizer
      日本農芸化学会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Remarks] 日本大学生物資源科学部食品生命学科食品分析学研究室

    • URL

      http://hp.brs.nihon-u.ac.jp/~foodanalysis/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究室の研究テーマ(食品廃棄物系バイオマスからの新規蛍光物質への変換プロセス)

    • URL

      http://hp.brs.nihon-u.ac.jp/~foodanalysis/research_subject/index.html#effective_resource

    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 蛍光物質及びその製造方法2017

    • Inventor(s)
      岩淵範之, 松藤寛, 砂入道夫, 坂野優稀
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-030189
    • Filing Date
      2017-02-21
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 蛍光物質及びその製造方法2016

    • Inventor(s)
      岩淵範之, 松藤寛, 砂入道夫, 坂野優稀
    • Industrial Property Rights Holder
      日本大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-160123
    • Filing Date
      2016-08-17
    • Related Report
      2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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