| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Most bacteria contain TA system which causes conditional growth inhibition and even may leads to cell death. The aim of this study is to develop a method to induce the effective bacterial cell death by controlling the expression of the TA genes.
First, I choose nine types of TA systems from the bacterial DNA sequence database, and then cloned the TA genes under the control of lac promoter in low and medium copy number plasmids. The growth inhibition effect on host Agrobacterium cells by regulation of each TA gene expression was evaluated. Finally, four TA systems were selected as the cell death-inducible TA systems in this bacteria. One or more of the TA systems were introduced into the chromosomal DNA in the bacteria, and their growth inhibition effects were confirmed in different experimental condition. These data suggest that the TA system could be applicable as a tool for the biological containment methodology that prevents leakage of the gene modified bacteria.
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