| Project/Area Number |
15K12263
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | Kanazawa University |
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Principal Investigator |
Endoh Hiroshi 金沢大学, 自然システム学系, 准教授 (20272932)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | テトラヒメナ / 分泌ベクター / シロアリ腸内共生ベン毛虫 / エンドグルカナーゼ / セロビオヒドラーゼ / セルロース分解 / シロアリ / 共生鞭毛虫 |
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| Outline of Final Research Achievements |
Construction of a novel transformation vector for expressing and secreting foreign cellulase genes in the ciliate Tetrahymena thermopile. This vector consists of 3 separate parts: 1) a cassette carrying drug resistant gene for screening after bombardment, 2) an expression cassette with a secretion signal derived from Tetrahymena cystein protease 4 gene, 3) flanking sequences at both sides of beta-tubulin gene for homologous recombination. Introducing 4 different genes (endoglucanase (EG) 5 and 7, cellobiohydrolase (CBH) and lignin esterase Cip2-like) from the termite symbiotic protists for cellulolysis into the vector, their activities were measured after induction of gene expression and subsequent secretion outside cells, respectively. EG5 and 7 were efficiently expressed, showing strong activity. Lignin esterase Cip2-like activity was slightly detected. No CBH activity has been confirmed, suggesting difficult folding due to the complicated protein structure.
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