| Project/Area Number |
15K12267
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Design and evaluation of sustainable and environmental conscious system
|
|---|
| Research Institution | Prefectural University of Kumamoto |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Kenji 近畿大学, 産業理工学部, 教授 (20236582)
|
|---|
| Research Collaborator |
WAKIDA Izumi
KURADOMI Yuki
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | ポリヒドロキシアルカン酸 / 生分解性プラスチック / 脂肪酸合成経路 / 二酸化炭素 / 共重合ポリエステル / コポリマー / PHA |
|---|
| Outline of Final Research Achievements |
The (R)-3HA-CoA ligase gene in Pseudomonas sp. 61-3 was cloned and identified. The gene was encoded the protein composed of 560 amino acids with a calculated molecular mass of 62 kDa. PHA synthase gene (phaC1) and the genes encoding the enzymes which provide medium-chain-length 3HA unit through fatty acid biosynthesis pathway were introduced into Ralstonia eutropha H16dC (phbC -disruptant of H16). The recombinant strains accumulated 5-10 wt% P(3HB-co-3HA) with 5-6 mol% 3HA fractions from fructose as the sole carbon source. Whereas, the recombinants synthesized less than 2 wt% P(3HB) homopolymer from carbon dioxide and 3HA units were not incorpotaed the polymer chain.
|
