Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Outline of Final Research Achievements |
I aimed to develop integrated analytical methods of carbohydrate, lipid and energy metabolism in mice by measuring energy expenditure and analyses of metabolism of stable and radioactive isotopes of palmitic acid and glucose. The feeding of fish oil suppressed the activities of enzymes related to fatty acid synthesis and stimulated those of enzymes related to β-oxidation in mice liver. However, total consumption of isotopically labeled palmitic acid and glucose and synthesis of fatty acids from labeled glucose were not altered by the feeding of fish oil. The results suggest that alteration of hepatic triacylglycerol concentration induced by fish oil feeding is related to the other factors than fatty acid synthesis and β-oxidation in the liver.
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