Research Project
Grant-in-Aid for Challenging Exploratory Research
The present study aims to develop some salt taste-enhancing compounds. We constructed Skn-1a knocked-out mice (S-KO mice) in which sweet, bitter and umami taste recepting cells disappeared and sour taste recepting cell activity was enhanced 3 times. The RNA-seq analysis of this mouse made us find several copies of a candidate ion channel expressed specifically in Type I cells. These genes included an ion channel (X-channel) which stained the taste buds of S-KO mice. This channel was expressed in humans as well. Also, this X-channel was a sodium channel. To know the physiological property of this channel, this was introduced into HEK 293 cell, which was then submitted to electrophisical experiment. However, the HEK 293 cell did not respond to sodium ion. This proposes the possibility that the cell is associated with the transport of some other ions. To analyze the phenotype of X-channel, we constructed the KO mice to design the target sequence.
All 2017 2016 2015
All Journal Article (16 results) (of which Int'l Joint Research: 3 results, Peer Reviewed: 16 results, Open Access: 15 results, Acknowledgement Compliant: 5 results) Presentation (44 results) (of which Int'l Joint Research: 7 results, Invited: 9 results)
EBioMedicine
Volume: 8 Pages: 60-71
10.1016/j.ebiom.2016.04.031
Mol. Nutr. Food Res.
Volume: 61 Issue: 2 Pages: 1-15
10.1002/mnfr.201600477
PLoS ONE
Volume: 11 Issue: 5 Pages: e0155386-e0155386
10.1371/journal.pone.0155386
Development
Volume: 143 Pages: 2012-2024
10.1242/dev.134817
J. Food Process. Preserv.
Volume: 41 Issue: 1 Pages: e12857-e12857
10.1111/jfpp.12857
Neurosci Lett
Volume: 617 Pages: 46-51
10.1016/j.neulet.2016.01.050
J. Food Sci.
Volume: 81 Issue: 2
10.1111/1750-3841.13194
Journal of Functional Foods
Volume: 13 Pages: 100-107
10.1016/j.jff.2014.12.029
Journal of Neurochemistry
Volume: 印刷中 Issue: 6 Pages: 806-814
10.1111/jnc.13073
Biosci. Biotechnol. Biochem.
Volume: 79 Issue: 11 Pages: 1893-1897
10.1080/09168451.2015.1042833
Volume: 10 Issue: 5 Pages: e0126921-e0126921
10.1371/journal.pone.0126921
World J. Gastroenterol.
Volume: 21 Issue: 35 Pages: 10091-10103
10.3748/wjg.v21.i35.10091
Scientific Reports
Volume: 5 Issue: 1 Pages: 12947-12947
10.1038/srep12947
Physiol. Genomics
Volume: 47 Issue: 8 Pages: 355-363
10.1152/physiolgenomics.00009.2015
Volume: 22 Issue: 7 Pages: e0132016-e0132016
10.1371/journal.pone.0132016
120005745670
J. Agric. Food Chem.
Volume: 63 Issue: 38 Pages: 8493-8500
10.1021/acs.jafc.5b03193