Investing the Mechanism of Salty Taste Reception and Response
Project/Area Number |
15K12334
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Eating habits
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Research Institution | The University of Tokyo |
Principal Investigator |
ABE Keiko 東京大学, 大学院農学生命科学研究科(農学部), 特任教授 (10151094)
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Co-Investigator(Renkei-kenkyūsha) |
ISHIMARU Yoshiro 東京大学, 大学院農学生命科学研究科, 特任准教授 (10451840)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 塩味受容機構 / 味細胞 / イオンチャネル / 電気生理 / ゲノム編集 / 食と栄養 |
Outline of Final Research Achievements |
The present study aims to develop some salt taste-enhancing compounds. We constructed Skn-1a knocked-out mice (S-KO mice) in which sweet, bitter and umami taste recepting cells disappeared and sour taste recepting cell activity was enhanced 3 times. The RNA-seq analysis of this mouse made us find several copies of a candidate ion channel expressed specifically in Type I cells. These genes included an ion channel (X-channel) which stained the taste buds of S-KO mice. This channel was expressed in humans as well. Also, this X-channel was a sodium channel. To know the physiological property of this channel, this was introduced into HEK 293 cell, which was then submitted to electrophisical experiment. However, the HEK 293 cell did not respond to sodium ion. This proposes the possibility that the cell is associated with the transport of some other ions. To analyze the phenotype of X-channel, we constructed the KO mice to design the target sequence.
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Report
(3 results)
Research Products
(60 results)
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[Journal Article] Catecholamines facilitate fuel expenditure and protect against obesity via a novel network of the gut-brain axis in transcription factor Skn-1-deficient mice.2016
Author(s)
Ushiama, S., Ishimaru, Y., Narukawa, M., Yoshioka, M., Kozuka, C., Watanabe, N., Tsunoda, M., Osakabe, N., Asakura, T., Masuzaki, H., and Abe, K.
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Journal Title
EBioMedicine
Volume: 8
Pages: 60-71
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Quantitative deviating effects of maple syrup extract supplementation on the hepatic gene expression of mice fed a high-fat diet.2016
Author(s)
Kamei, A., Watanabe, Y., Shinozaki, F., Yasuoka, A., Shimada, K., Kondo, K., Ishijima, T., Toyoda, T., Arai, S., Kondo, T., and Abe, K.
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Journal Title
Mol. Nutr. Food Res.
Volume: 61
Issue: 2
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A high phosphorus diet affects lipid metabolism in rat liver: a DNA microarray analysis.2016
Author(s)
Chun, S., Bamba, T., Suyama, T., Ishijima, T., Fukusaki, E., Abe, K., and Nakai, Y.
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Journal Title
PLoS ONE
Volume: 11
Issue: 5
Pages: e0155386-e0155386
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Diversity in cell motility reveals the dynamic nature of the formation of zebrafish taste sensory organs.2016
Author(s)
Soulika, M., Kaushik, A-L., Mathieu, B., Lourenco, R., Komisarczuk, A.Z., Romano, S.A., Jouary, A., Lardennois, A., Tissot, N., Okada, S., Abe, K., Becker, T.S., and Kapsimali, M.
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Journal Title
Development
Volume: 143
Pages: 2012-2024
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Effect of proteolytic modification on texture and mastication of heat-treated egg white gels.2016
Author(s)
Funaki, J., Minami, M., Abe, S., Ueda, R., Eto, W., Kugino, K., Kugino, M., Abe, K., Toko, K., and Asakura, T.
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Journal Title
J. Food Process. Preserv.
Volume: 41
Issue: 1
Pages: e12857-e12857
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Expression of serotonin receptor genes in cranial ganglia.2016
Author(s)
Maeda, N., Ohmoto, M., Yamamoto, K., Kurokawa, A., Narukawa, M., Ishimaru, Y., Misaka, T., Matsumoto, I., Abe, K.
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Journal Title
Neurosci Lett
Volume: 617
Pages: 46-51
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Preparation of an Aroma Fraction from Dried Bonito by Steam Distillation and Its Effect on Modification of Salty and Umami Taste Qualities.2016
Author(s)
Ogasawara, Y., Mochimaru, S., Ueda, R., Ban, M., Kabuto, S., and Abe, K.
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Journal Title
J. Food Sci.
Volume: 81
Issue: 2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Administration of a maple syrup extract to mitigate their hepatic inflammation induced by a high-fat diet: a transcriptome analysis.2015
Author(s)
Kamei, A., Watanabe, Y., Shinozaki, F., Yasuoka, A., Kondo, T., Ishijima, T., Toyoda, T., Arai, S., and Abe, K.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 79
Issue: 11
Pages: 1893-1897
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury.2015
Author(s)
Yoshida, K., Ushida, Y., Ishijima, T., Suganuma, H., Inakuma, T., Yajima, N., Abe, K., and Nakai, Y.
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Journal Title
World J. Gastroenterol.
Volume: 21
Issue: 35
Pages: 10091-10103
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of key neoculin residues responsible for the binding and activation of the sweet taste receptor.2015
Author(s)
Koizumi, T., Terada, T., Nakajima, K., Kojima, M., Koshiba, S., Matsumura, Y., Kaneda, K., Asakura, T., Shimizu, A., Abe, K., and Misaka, T.
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 12947-12947
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Effect of orally administered collagen hydrolysate on gene expression profiles in mouse skin: a DNA microarray analysis.2015
Author(s)
Oba, C., Ito, K., Ichikawa, S., Morifuji, M., Nakai, Y., Ishijima, T., Abe, K., and Kawahata, K.
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Journal Title
Physiol. Genomics
Volume: 47
Issue: 8
Pages: 355-363
DOI
Related Report
Peer Reviewed
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[Journal Article] Rapid Expansion of Phenylthiocarbamide Non-Tasters among Japanese Macaques.2015
Author(s)
Suzuki-Hashido, N., Hayakawa, T., Matsui, A., Go, Y., Ishimaru, Y., Misaka, T., Abe, K., Hirai, H., Satta, Y., and Imai, H.
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Journal Title
PLoS ONE
Volume: 22
Issue: 7
Pages: e0132016-e0132016
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Long-Chain Fatty Acids Elicit a Bitterness-Masking Effect on Quinine and Other Nitrogenous Bitter Substances by Formation of Insoluble Binary Complexes.2015
Author(s)
Ogi, K., Yamashita, H., Terada, T., Homma, R., Shimizu-Ibuka, A., Yoshimura, E., Ishimaru, Y., Abe, K., and Asakura, T.
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Journal Title
J. Agric. Food Chem.
Volume: 63
Issue: 38
Pages: 8493-8500
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Loss of brush cells and taste cells in the gastrointestinal tract alters energy metabolism in Skn-1- deficient mice2015
Author(s)
Ishimaru, Y., Ushiama, S., Narukawa, M., Yoshioka, M., Kozuka, C., Watanabe, N., Osakabe, N., Asakura, T., Masuzaki, H., and Abe, K.
Organizer
ECRO2015 European Chemoreception Research Organization
Place of Presentation
Istanbul, Republic of Turkey
Year and Date
2015-09-02
Related Report
Int'l Joint Research
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[Presentation] DNA microarray analysis of labial salivary glands in patients with Sjögren’s syndrome: comparison with IgG4-related disease2015
Author(s)
Takahashi, H., Tsuboi, H., Nakai, Y., Iizuka, M., Asashima, H., Hirota, T., Kondo, Y., Moriyama, M., Furukawa, S., Matsumoto, I., Nakamura, S., Yoshihara, T., Abe, K., and Sumida, T.
Organizer
The 13th International Symposium on Sjögren's Syndrome (ISSS, Bergen)
Place of Presentation
Bergen, Norway
Year and Date
2015-05-19
Related Report
Int'l Joint Research
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