Functional analysis for lifestyle diseases-related molecules based on molecular epidemiologic study

• Project/Area Number: 15K12342
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Nagasaki University
• Principal Investigator: MORI Ryoichi 長崎大学, 医歯薬学総合研究科(医学系), 准教授 (30509310)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: miRNA / Foxo / カロリー制限 / 寿命 / 代謝

Outline of Final Research Achievements

MicroRNAs (miRNAs) of the small, non-coding RNA family that regulate translational processing of target mRNAs. miRNAs in fluid might be associated with diseases and application to biomarker. However molecular and cellular mechanisms are largely unknown. In this study, we have identified lifestyle diseases-related miR-22-3p using molecular epidemiologic study. And we clarified that age-related transcription factor Foxo3a regulates expression level of miR-22-3p in serum.

Research Products

• [Journal Article] Neuropeptide Y resists excess loss of fat by lipolysis in calorie-restricted mice: A trait potential for the life-extending effect of calorie restriction (2017)
  • Author(s): Park S, Komatsu T, Kim SE, Tanaka K, Hayashi H, Mori R, Shimokawa I
  • Journal Title: Aging Cell Volume: 16 Issue: 2 Pages: 339-348
  • DOI: 10.1111/acel.12558
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Potency of umbilical cord blood- and Wharton’s jelly-derived mesenchymal stem cells for scarless wound healing (2015)
  • Author(s): Doi H., Kitajima Y., Luo L., Yan C., Tateishi S., Ono Y., Urata Y., Goto S., Mori R., Masuzaki H., Shimokawa I., Hirano A. & Li T. S.
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 18844-18844
  • DOI: 10.1038/srep18844
  • Peer Reviewed / Open Access
• [Journal Article] Ephrin-Bs drive junctional downregulation and actin stress fiber disassembly to enable wound re-epithelialisation (2015)
  • Author(s): Nunan R., Campbell J., Mori R., Pitulescu M. E., Jiang W. G., Harding K. G. , Adams H. G., Nobes C. D. & Martin P.
  • Journal Title: Cell Rep Volume: 13 Issue: 7 Pages: 1380-1380
  • DOI: 10.1016/j.celrep.2015.09.085
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Upregulation of cytochrome c oxidase subunit 6b1 (Cox6b1) and formation of mitochondrial supercomplexes: implication of Cox6b1 in the effect of calorie restriction. (2015)
  • Author(s): Kim S. E., Mori R., Komatsu T., Chiba T., Hayashi H., Park S., Sugawa M. D., Dencher N. A. & Shimokawa I.
  • Journal Title: Age (Dordr) Volume: 37 Issue: 3 Pages: 9787-9787
  • DOI: 10.1007/s11357-015-9787-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The life-extending effect of dietary restriction requires Foxo3 in mice (2015)
  • Author(s): Shimokawa I, Komatsu T, Hayashi N, Kim SE, Kawata T, Park S, Hayashi H, Yamaza H, Chiba T, Mori R
  • Journal Title: Aging Cell Volume: - Issue: 4 Pages: 707-707
  • DOI: 10.1111/acel.12340
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of the adiponectin gene mimics the metabolic and stress resistance effects of calorie restriction, but not the anti-tumor effect (2015)
  • Author(s): Kamohara R, Yamaza H, Tsuchiya T, Komatsu T, Park S, Hayashi H, Chiba T, Mori R, Otabe S, Yamada K, Nagayasu T, Shimokawa I
  • Journal Title: Experimental Gerontology Volume: 64 Pages: 46-54
  • DOI: 10.1016/j.exger.2015.02.011
  • NAID: 120006987178
  • Peer Reviewed / Open Access
• [Presentation] 創傷治癒における microRNA の新機能 (2016)
  • Author(s): 森 亮一
  • Organizer: 第46回日本創傷治癒学会
  • Place of Presentation: 東京大学（東京都）
  • Year and Date: 2016-12-09
  • Invited
• [Presentation] Identification of wound inflammation-related miRNAs: miR-223-deficient neutrophil improve Staphylococcus aureus-infected skin wound sites (2016)
  • Author(s): Mori R
  • Organizer: EMBO conferences: The molecular and cellular basis of regeneration and tissue repair
  • Place of Presentation: Salerno (Italy)
  • Year and Date: 2016-09-17
  • Int'l Joint Research
• [Presentation] 皮膚瘢痕形成の分子メカニズム解明および核酸医薬開発への応用 (2016)
  • Author(s): 森 亮一
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-05-12
• [Presentation] 皮膚創傷治癒過程における miR-223 の機能解析 (2015)
  • Author(s): 森 亮一
  • Organizer: 第45回 日本創傷治癒学会総会
  • Place of Presentation: JPタワーホール＆カンファレンス（東京都千代田区）
  • Year and Date: 2015-11-30
• [Presentation] miR-223 regulates neutrophil activation in the acute inflammatory response during skin wound healing (2015)
  • Author(s): 森 亮一
  • Organizer: Gordon Research Conference (Tissue Repair & Regeneration)
  • Place of Presentation: New London (USA)
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] 組織修復及び炎症制御に関与する microRNA の病態生理学的解析 (2015)
  • Author(s): 森 亮一
  • Organizer: 第１０４回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-04-30
• [Book] Role of the forkhead box O family and neuropeptide Y in calorie restriction. Aging Mechanisms: Longevity, Metabolism, and Brain Aging (2015)
  • Author(s): Mori R, Park S. & Shimokawa I
  • Total Pages: 430
  • Publisher: Springer