Development of a new ambient ionization-ion mobility spectrometric method for detecting fungal species in early growth stages
| Project/Area Number |
15K12446
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cultural assets study and museology
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| Research Institution | Nara Women's University |
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Principal Investigator |
Takeuchi Takae 奈良女子大学, 自然科学系, 准教授 (80201606)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 微生物由来揮発性有機化合物 / 真菌 / アンビエントイオン化質量分析 / DART / セスキテルペン / イオン移動度スペクトロメトリー / MVOC / カビ代謝物質 / アンビエントイオン化法 / 真菌代謝物質 / リアルタイムオンサイト分析 / 精密質量測定 / イオンモビリティ / カビ臭物質モニタリング / 質量分析 / オンサイト分析 / 文化財 / DARTイオン化 / アンビエントイオン化 / リアルタイム直接分析 |
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| Outline of Final Research Achievements |
Fungi occurring in cultural properties and the environment are problematic. Microbial volatile organic compounds (MVOC) emitted from fungi can be used as a notification of fungal growth in the environment. In this study, we developed a Direct Analysis in Real Time (DART) MVOC monitoring system. MVOC emitted from Penicillium paneum, Fusarium solani, Aspergillus fumigatus, and Aspergillus nidulans in the head-space of the flasks were directly analyzed using the DART-Orbitrap mass spectrometer. The characteristic peaks which correspond to [M-H]+ ions and [M+H]+ ions of C15H24 appeared in the DART mass spectra of MVOCs from Penicillium paneum and Aspergillus fumigatus with the high mass resolution mode. The peak at m/z 123.0447 in the DART mass spectrum of P. paneum was assigned to benzoic acid. It was concluded that the MVOC monitoring with the DART ion source is a promising method for identifying fungal species.
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Report
(4 results)
Research Products
(23 results)
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[Presentation] 糖ペプチドのイオンモビリティ―質量分析法2015
Author(s)
田尻道子, 竹内孝江, 本郷弥生, 中村健道, 廣瀬賢治, 和田芳直
Organizer
第63回質量分析総合討論会,2015,つくば
Place of Presentation
茨城県つくば市、つくば国際会議場エポカルつくば
Year and Date
2015-06-18
Related Report
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