• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Challenge for development of placenta-on-a-chip

Research Project

Project/Area Number 15K12500
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTohoku University

Principal Investigator

Funamoto Kenichi  東北大学, 学際科学フロンティア研究所, 准教授 (70451630)

Research Collaborator YOSHINO Daisuke  東北大学, 学際科学フロンティア研究所, 助教
ITO Takuya  自治医科大学, 先端医療技術開発センター, 講師
SUGIBAYASHI Rika  国立成育医療研究センター, 周産期・母性診療センター, 医員
FUNAMOTO Kiyoe  東北大学, 大学院医学系研究科, 技術補佐員
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsマイクロ・ナノデバイス / 細胞・組織 / 生物・生体工学 / 流体工学 / ナノバイオ / マイクロ流体デバイス / 胎盤 / 低酸素
Outline of Final Research Achievements

A placenta-on-a-chip was developed in this study in order to reproduce the structure and function of the microenvironment of a human placenta. Media channels, which model intervillous space and fetal blood vessel, were placed flanking the three parallel gel channels which mimic the extracellular matrix. Gas channels through which gas mixtures are supplied to control the oxygen tension inside the chip were placed above the media channels. A method to form monolayers of human choriocarcinoma cells (JEG3s) and human umbilical vein endothelial cells (HUVECs) in the media channels, and a method to measure the permeability of the monolayers were established. The co-culture of JEG3s and HUVECs under a controlled oxygen tension at different concentrations showed variations of cell morphology and cell junctions, implying that oxygen tension can influence the cell-cell interaction.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] マサチューセッツ工科大学(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] マサチューセッツ工科大学(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Endothelial monolayer permeability under controlled oxygen tension2017

    • Author(s)
      Funamoto, K; Yoshino, D; Matsubara, K; Zervantonakis, I; Funamoto, K; Nakayama, M; Masamune, J; Kimura, J; Roger, K
    • Journal Title

      Integrative Biology

      Volume: 6 Issue: 6 Pages: 529-538

    • DOI

      10.1039/c7ib00068e

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 低酸素負荷による血管内皮細胞単層の物質透過性と接着結合の変化2017

    • Author(s)
      船本健一,吉野大輔,松原健人,船本聖絵,木村芳孝,Roger D. Kamm
    • Organizer
      日本機械学会第29回バイオエンジニアリング講演会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      2017-01-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 低酸素ストレスに対する血管内皮細胞単層の細胞間結合の初期応答2017

    • Author(s)
      船本健一,吉野 大輔,中山 勝文
    • Organizer
      日本機械学会第30回バイオエンジニアリング講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Microfluidic experiments of cellular responses to hypoxia2016

    • Author(s)
      Kenichi Funamoto
    • Organizer
      The 13th International Conference on Flow Dynamics (ICFD2016)
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-10-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited

URL: 

Published: 2015-04-16   Modified: 2022-06-07  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi