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Study of high-throughput single molecular tensile testing for binding force spectrum and application to bio-analysis

Research Project

Project/Area Number 15K12505
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionUniversity of Yamanashi

Principal Investigator

UKITA Yoshiaki  山梨大学, 総合研究部, 助教 (40578100)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords生体分子間相互作用 / 分子引っ張り試験 / 分子間相互作用
Outline of Final Research Achievements

This study explored a molecular tensile testing method that able to measure the mechanical force characterization of bio-active molecules, such as antibody. Antibody-immobilized microbeads and molecular testing platforms were developed. The measurements of binding force between Mouse IgG and anti-MouseIgG antibody were carried out based on the developed testing platform. By the series of investigations, we obtained the molecular binding force extending from few 10 pN to 100 pN, which is consistent with the previously reported binding forces of biomolecule measured by atomic force microscopy. Therefore it is concluded that the proposed method is successfully established.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2016

All Presentation (3 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] ハイスループット生体分子間相互作用測定法のための抗体固定化基板の開発2017

    • Author(s)
      大竹真央,浮田芳昭
    • Organizer
      第64回応用物理学会春季学術講演会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Proposal of Molecular Tensile Testing by a Centrifugal Microfluidic Approach2016

    • Author(s)
      M. Otake, Y. Ukita
    • Organizer
      29th International Microprocesses and Nanotechnology Conference (MNC2016)
    • Place of Presentation
      ANAクラウンプラザ京都(京都府・京都市)
    • Year and Date
      2016-11-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 遠心マイクロ流体デバイスを用いた生体分子間相互作用測定法の提案2016

    • Author(s)
      大竹真央,浮田芳昭
    • Organizer
      山梨講演会2016
    • Place of Presentation
      山梨大学甲府キャンパス(山梨県・甲府市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 分子計測法およびそれを用いた分子計測装置2016

    • Inventor(s)
      浮田芳昭
    • Industrial Property Rights Holder
      国立大学法人山梨大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-192894
    • Filing Date
      2016-09-30
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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