| Project/Area Number |
15K12524
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FUKUDA Norio 東京慈恵会医科大学, 医学部, 准教授 (30301534)
OYAMA Kotaro 東京慈恵会医科大学, 医学部, 訪問研究員 (70632131)
SHIMOZAWA Togo 東京大学, 理学部技術部, 技術専門職員 (00386608)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 熱パルス / in vivoイメージング / サルコメア / 心筋細胞 / 熱 / 心筋症 / 心筋 / ナノイメージング / 心筋収縮 / カルシウムイオン / 拡張性心筋症 |
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| Outline of Final Research Achievements |
In the present study, we developed a high-speed (100 fps), high-resolution (20 nm) confocal microscopic system enabling simultaneous imaging of sarcomere length (SL) and the intracellular Ca concentration ([Ca]i) in left ventricular cardiomyocytes in mice in vivo. During imaging, we measured macroscopic parameters such as electrocardiogram, left ventricular pressure and the left ventricular pressure-volume relationship, simultaneous with SL and [Ca]i. By using this system, we investigated the effects of pulsed infra-red laser irradiation on cardiomyocytes labeled for the T-tubules, and found that myocyte contractions were enhanced. These findings indicate that infra-red laser irradiation is useful in promoting myocardial contraction in vivo.
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