Very rapid DNA Amplification system using vibration method
Project/Area Number |
15K12525
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
|
Research Institution | Kanagawa University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | 核酸 / 増幅 / 振動 / 周波数 / DNA / PCR / ペルチェ |
Outline of Final Research Achievements |
We studied on the fabrication of very rapid polymerase chemical reaction (PCR) system without the conventional thermal cycle, which generally damages DNA and enzymes. To overcome the problem, we have been targetting the development of very rapid PCR system performing with speed per second. In this study, we used a vibration method which we discovered previously, and a vibration unit composed of a coil and magnets was used. We examined DNA amplifcation on the condition that vibration frequency ranged from 100Hz to 1000Hz and applied voltage was altered from 0V to 20V. Electrophoresis and quantative evaluation using Multina system revealed that our template rambda DNA was amplified at over 170Hz.
|
Report
(3 results)
Research Products
(5 results)
-
-
-
[Presentation] 振動法によるDNA増幅2016
Author(s)
松下岳史,鈴木温,井上和仁,安積良隆,山口栄雄
Organizer
電子情報通信学会総合大会
Place of Presentation
九州大学
Year and Date
2016-03-15
Related Report
-
-