| Project/Area Number |
15K12527
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomedical engineering/Biomaterial science and engineering
|
|---|
| Research Institution | Sojo University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
市原 英明 崇城大学, 生物生命学部, 准教授 (70369114)
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | リウマチ / アポトーシス / リポソーム / 炎症性サイトカイン |
|---|
| Outline of Final Research Achievements |
We have produced hybrid liposomes(HL)which can be prepared by sonication of a mixture of vesicular and micellar molecules in a buffer solution. We investigated the anti- rheumatoid arthritis (RA) effects of HL and following results are obtained.(A)The remarkable inhibitory effects of HL on the growth of human RA fibroblast-like synoviocytes (HFLS-RA) and human synovial sarcoma (SW982)cells were attained in vitro.(B) HL induced apoptotic death of HFLS-RA and SW982 cells through the activation of caspase-3, -8 and -9. (C) Therapeutic effects of HL with downregulation of inflammatory cytokine （TNF-α、IL-6 and IL-1β）for the mouse model with RA arthritis were obtained in vivo. These findings suggest that HL showed inhibitory effects on the growth of HFLS-RA and SW982 cells in vitro along with apoptosis and therapeutic effects for mouse models of RA in vivo.
|
