| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In this study, we tried to develop the new transfection device based on the nanofiber-cell strong interaction and using the 1D continuity of fiber and chemotaxis of the cell. Flow chamber system utilized FBS as chemotactic factor was developed. Using the above system, gene introduction to GFP-Hela cell on a filament was evaluated by analyzing the quenching of GFP by using GFP-siRNA as transgene. In the current status, cell spreading on the filamental substrate and quenching of the GFP were observed, but, apotosis was also observed, targeted high efficiency system is not developed yet. We will push forward further examination in future.
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