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Investigation of the mechanism of gene transfer by sonoporation for fetus

Research Project

Project/Area Number 15K12530
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionNational Institute of Maritime, Port and Aviation Technology (2016)
Port and Airport Research Institute (2015)

Principal Investigator

SATO Tomoo  国立研究開発法人海上・港湾・航空技術研究所, 港湾空港技術研究所, 専任研究員 (10242918)

Co-Investigator(Kenkyū-buntansha) 千葉 敏雄  日本大学, 総合科学研究所, 教授 (20171944)
宮本 義孝  国立研究開発法人国立成育医療研究センター, 細胞医療研究部, 共同研究員 (20425705)
黒川 量雄  国立研究開発法人理化学研究所, 光量子工学研究領域, 専任研究員 (40333504)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsソノポレーション / 胎児治療 / 遺伝子導入 / 超音波 / マイクロバブル / 実験計画法 / 緑色蛍光タンパク質 / 蛍光画像 / 高感度高速共焦点顕微鏡
Outline of Final Research Achievements

There is a great concern about the gene therapy to fetuses. We pay attention to sonoporation which is method using ultrasound and micro-bubbles to transfer the gene into target organ (cell) selectively. The purpose of our study is to build the method of gene-transfer that is more efficiently and more safety, by studying the dynamics of the cell membrane during sonoporation. We tried development of the technique to observe the dynamics of the cell membrane using super high spatio-temporal resolution confocal microscope with high sensitivity.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 2015 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (4 results)

  • [Journal Article] Sar1 localizes at the rims of COPII-coated membranes in vivo.2016

    • Author(s)
      Kazuo Kurokawa, Yasuyuku Suda and Akihiko Nakano
    • Journal Title

      J. Cell Sci.

      Volume: 129 Issue: 17 Pages: 3231-3237

    • DOI

      10.1242/jcs.189423

    • NAID

      120007129359

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] COPI is essential for Golgi cisternal maturation and dynamics.2016

    • Author(s)
      Midori Ishii, Yasuyuki Suda, Kazuo Kurokawa, and Akihiko Nakano
    • Journal Title

      J. Cell Sci.

      Volume: 129 Issue: 17 Pages: 3251-3261

    • DOI

      10.1242/jcs.193367

    • NAID

      120007129358

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ゴルジ体によるタンパク質輸送機構2016

    • Author(s)
      黒川量雄,中野明彦
    • Journal Title

      生物物理

      Volume: 56 (4) Pages: 201-206

    • NAID

      130005165634

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Live-cell visualization of excitation energy dynamics in chloroplast thylakoid structures.2016

    • Author(s)
      Masakazu Iwai, Makio Yokono, Kazuo Kurokawa, Akira Ichihara, and Akihiko Nakano
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 29940-29940

    • DOI

      10.1038/srep29940

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Fetal gene therapy for ornithine transcarbamylase deficiency by intrahepatic plasmid DNA-microbubble injection combined with hepatic ultrasound insonation2016

    • Author(s)
      Oishi Y, Kakimoto T, Yuan W, Kuno S, Yamashita H, Chiba T
    • Journal Title

      Ultrasound in Medicine & Biology

      Volume: 42 Pages: 1357-61

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 超音波遺伝子導入の光学的実時間観察のための超音波照射条件の実験計画法を用いた検討2017

    • Author(s)
      佐藤智夫,山下紘正,宮本義孝,黒川量雄,千葉敏雄
    • Organizer
      電子情報通信学会超音波研究会
    • Place of Presentation
      東京
    • Year and Date
      2017-06-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 培養デバイス TASCL を用いたヒト脂肪様細胞組織体の創製2017

    • Author(s)
      宮本義孝, 池内真志, 野口洋文, 鈴木聡, 八木透, 生田幸士, 林衆治
    • Organizer
      シンポジウム:細胞アッセイ技術の現状と将来
    • Place of Presentation
      東京
    • Year and Date
      2017-01-31
    • Related Report
      2016 Annual Research Report
  • [Presentation] 3次元培養によるヒト脂肪由来幹細胞の脂肪への分化とマイクロティッシュの作製2016

    • Author(s)
      宮本義孝, 池内真志, 野口洋文, 八木透, 生田幸士, 林衆治
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 4D imaging of membrane trafficking in living Saccharomyces cerevisiae2016

    • Author(s)
      Kazuo Kurokawa
    • Organizer
      14th International Congress on Yeasts
    • Place of Presentation
      淡路
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 小胞体ゴルジ体間、ゴルジ体槽間のタンパク質輸送のイメージング2016

    • Author(s)
      黒川量雄
    • Organizer
      日本遺伝学会第88回大会
    • Place of Presentation
      静岡
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 積荷タンパク質のゴルジ体槽間輸送機構2016

    • Author(s)
      黒川量雄,石井みどり,中野明彦
    • Organizer
      第68回日本細胞生物学会大会
    • Place of Presentation
      京都
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Sar1 concentrates at the neck of COPII-coated vesicle in vivo2015

    • Author(s)
      黒川量雄,須田恭之,中野明彦
    • Organizer
      第38回日本分子生物学会年会/第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートピアアイランド(兵庫)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Sar1 concentrates on the neck region of COPII-coated vesicle at the endoplasmic reticulum exit sites in vivo2015

    • Author(s)
      黒川量雄,中野明彦
    • Organizer
      Cell biology of yeasts
    • Place of Presentation
      Cold spring Harbor laboratory (New York)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Studies on the molecular mechanism of cisternal maturation in budding yeast Sacharomyces cerevisiae2015

    • Author(s)
      石井みどり,黒川量雄,須田恭之,中野明彦
    • Organizer
      Cell biology of yeasts
    • Place of Presentation
      Cold spring Harbor laboratory (New York)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞内膜交通の高速高感度高解像イメージング2015

    • Author(s)
      黒川量雄,中野明彦
    • Organizer
      第67日本細胞生物学会大会
    • Place of Presentation
      タワーホール船橋(東京)
    • Year and Date
      2015-07-02
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 培養デバイスTASCLを用いた肝細胞組織体の創製2015

    • Author(s)
      宮本義孝, 池内真志, 野口洋文, 鈴木聡, 八木透, 生田幸士, 林衆治
    • Organizer
      第22 回HAB研究機構学術年会
    • Place of Presentation
      昭和大(東京)
    • Year and Date
      2015-06-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 培養デバイスTASCLによる初代肝細胞スフェロイド培養、および均一・大量生産系の確立2015

    • Author(s)
      宮本義孝, 池内真志, 野口洋文, 鈴木聡, 八木透, 生田幸士, 林衆治
    • Organizer
      第54回日本生体医工学会大会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2015-05-07
    • Related Report
      2015 Research-status Report
  • [Remarks] 理科科学研究所 光量子工学研究領域 生細胞超解像イメージング研究チーム

    • URL

      http://www.riken.jp/research/labs/rap/extr_photonics/live_cell_superresolution_img/

    • Related Report
      2016 Annual Research Report
  • [Remarks] Sar1の生細胞内三次元局在を可視化 -積荷タンパク質輸送小胞の鍵分子の限局を観察-

    • URL

      http://www.riken.jp/pr/press/2016/20160902_1/

    • Related Report
      2016 Annual Research Report
  • [Remarks] ゴルジ体槽成熟の分子機構を解明 -COPⅠ被覆タンパク質の機能が必須-

    • URL

      http://www.riken.jp/pr/press/2016/20160902_2/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 躍動する光合成反応を可視化 -超解像イメージングで生きた細胞の光エネルギー伝達を観察-

    • URL

      http://www.riken.jp/pr/press/2016/20160715_1/

    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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