| Project/Area Number |
15K12547
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KIKUCHI Akihiko 東京理科大学, 基礎工学部, 教授 (40266820)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 光応答性 / フルオロポリマー / スピロベンゾピラン / コーティング / 細胞接着制御 / スピロピラン / 疎水性表面 / 細胞接着 |
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| Outline of Final Research Achievements |
Glass surfaces coated with spirobenzopyran-installed fluoroalkyl polymer was prepared to control cell adhesion behavior through the surface hydrophobic alternation derived from spirobenzopyran photoisomerization between high polar merocyanine and low polar spiropyran form. Polymer-coated surface exhibited the reversible change of adsorption spectra and static contact angle change which were attributed to the photoisomerization by switching ultraviolet(UV)/visible(Vis) light.
Bovine endothelial cells greatly adhered on the moderately hydrophobic surface with UV treatment, compared to the UV-treated strongly hydrophobic surface. Adhering cells spontaneously detached after Vis treatment. Moreover, confluently cultured cells also peeled off as a sheet-like cell structure with maintaining cell-cell junctions and extracellular matrix proteins.
The concept of light-responsive surface would be a unique strategy for cell manipulation in applications of cell-based therapies.
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