| Project/Area Number |
15K12567
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical engineering assessment
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川瀬 知之 新潟大学, 医歯学系, 准教授 (90191999)
永田 昌毅 新潟大学, 医歯学系, 准教授 (10242439)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 再生治療 / 移植治療用細胞 / 酸化ストレス / DNA傷害 / 間葉系幹細胞 / 骨膜細胞 / 細胞形態 / 細胞品質管理 / 移植用細胞 / 品質管理 / 酸化ストレス刺激細胞 / 遺伝的不安定性細胞 / DNA傷害マーカー / リン酸化H2AX / p53 |
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| Outline of Final Research Achievements |
The aim was to examine the efficient and quantitative method to assure the quality of cells to be used in cell therapy. Focused on the DNA damage markers, it was analyzed the movement of the cell-surface marker, the cell adhesion factor, the growth receptor and the protein related cell cycle using the flow cytomery (FCM) or the immunofluorescent staining. It was demonstrated that γ-ray-irradiation suppressed proliferation, enlarged cells and cell nuclei, and upregulated γ-H2AX. However, when observing the living cell which irradiated a γ-beam with the digital holographic microscope (DHM), there was a change only in the indexes related to cell size and the marker which is related to DNA damage repair were not substantially upregulated. Instead of DNA damage markers, we suggest that cell morphological parameters that are monitored by DHM could be a useful for the cell quality control.
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