| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
To uncover the molecular mechanisms of myoblast fusion events, we firstly established a novel assay system to monitor the cell fusion of cultured myoblast C2C12 cells. Using this system, we searched for new genes and compounds that affects the cell fusion of C2C12, and GGA1, a clathrin adaptor commonly expressed in eukaryotic cells was identified. GGA1 expression was found to be up-regulated during myogenesis of C2C12. Silencing of GGA1 caused reduction of myotube formation and we found that depletion of GGA1 caused decrease of cell surface expression of insulin receptor. We also identified a compound that has a potential to inhibit myogenesis and its molecular action is under investigation.
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