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Hunting of novel myokines by RNAseq

Research Project

Project/Area Number 15K12668
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Sports science
Research InstitutionTokyo Metropolitan University

Principal Investigator

Fujii Nobuharu L  首都大学東京, 人間健康科学研究科, 教授 (40509296)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords骨格筋 / RNAseq / マイオカイン / 次世代シーケンス / 転写物
Outline of Final Research Achievements

Messenger RNA obtained from human skeletal muscle tissue was provided to Next Generation Sequence (RNAseq) to identify gene transcripts exhaustively. The transcripts were compared to human genome information to seek transcripts that had not reported yet. Then, secretion protein-like molecules were estimated from amino acid sequence of the transcripts. Finally, we found 3 secretion protein-like molecules expressed in skeletal muscle. One of the 3 was focused in the subsequent study. Unfortunately we did not achieve susses to make a specific antibody for the molecule. Therefore HA-tagged expression vector of the molecule was generated and expressed in cultured skeletal muscle cells. The expressed protein was detected in conditioned culture medium by Western blotting used HA-antibody. These results suggest that the molecule is a novel myokine secured from skeletal muscle.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (31 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 11 results,  Open Access: 9 results,  Acknowledgement Compliant: 6 results) Presentation (13 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Arnold School of Public Health/Applied Physiology Division/University of South Carolina(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Role of satellite cells in skeletal muscle plasticity, beyond muscle regeneration.2017

    • Author(s)
      Tamura K, Furuichi Y, ManabeY, Fujii NL
    • Journal Title

      J Phys Fitness Sports Med

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An improved glucose transport assay system for isolated mouse skeletal muscle tissues2016

    • Author(s)
      Inagaki A, Maruo K, Furuichi Y, Miyatake S, Tamura K, Fujii NL, Manabe Y
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 80 Issue: 11 Pages: 2224-2230

    • DOI

      10.1080/09168451.2016.1210503

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of 17β-Estradiol and Androgen on Glucose Metabolism in Skeletal Muscle2016

    • Author(s)
      Inada A, Fujii NL, Inada O, Higaki Y, Furuichi Y, Nabeshima YI.
    • Journal Title

      Endocrinology

      Volume: 157 Issue: 12 Pages: 4691-4705

    • DOI

      10.1210/en.2016-1261

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Adjusting the 17β-Estradiol-to-Androgen Ratio Ameliorates Diabetic Nephropathy.2016

    • Author(s)
      Inada A, Inada O, Fujii NL, Nagafuchi S, Katsuta H, Yasunami Y, Matsubara T, Arai H, Fukatsu A, Nabeshima YI.
    • Journal Title

      J Am Soc Nephrol.

      Volume: 27 Issue: 10 Pages: 3035-3050

    • DOI

      10.1681/asn.2015070741

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes2016

    • Author(s)
      Manabe Y, Ogino S, Ito M, Furuichi Y, Takagi M, Yamada M, Goto-Inoue N, Ono Y, Fujii NL
    • Journal Title

      Anal Biochem

      Volume: 497 Pages: 36-38

    • DOI

      10.1016/j.ab.2015.10.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Experimental models research on skeletal muscle contraction2016

    • Author(s)
      Manabe Y, Fujii NL
    • Journal Title

      J Phys Fit Sport Med

      Volume: 5 Pages: 373-377

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes2016

    • Author(s)
      Manabe Y, Ogino S, Ito M, Furuichi Y, Takagi M, Yamada M, Goto-Inoue N, Ono Y, Fujii NL.
    • Journal Title

      Anal Biochem.

      Volume: 497 Pages: 36-38

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction2015

    • Author(s)
      Goto-Inoue N, Tamura K, Motai F, Ito M, Miyata K, Manabe Y, Fujii NL
    • Journal Title

      Mol Cell Biochem

      Volume: 411 Issue: 1-2 Pages: 173-180

    • DOI

      10.1007/s11010-015-2579-8

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Contraction stimulates muscle glucose uptake independent of atypical PKC2015

    • Author(s)
      Yu H,Fujii NL,Toyoda T, An D, Farese RV,Leitges M, Hirshman MF, Mul JD, Goodyear LJ.
    • Journal Title

      Physiol Rep

      Volume: 3 Issue: 11 Pages: e12565-e12565

    • DOI

      10.14814/phy2.12565

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Early pathogenesis of Duchenne muscular dystrophy modelled in patient-derived human induced pluripotent stem cells2015

    • Author(s)
      Shoji E, Sakurai H, Nishino T, Nakahata T, Heike T, Awaya T, Fujii N, Manabe Y, Matsuo M, Sehara-Fujisawa A.
    • Journal Title

      Sci Rep.

      Volume: 5 Pages: 12831-12831

    • NAID

      120005649326

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A fragmented form of annexin A1 is secreted from C2C12 myotubes by electric pulse-induced contraction.2015

    • Author(s)
      Goto-Inoue N, Tamura K, Motai F, Ito M, Miyata K, Manabe Y, Fujii NL.
    • Journal Title

      Mol Cell Biochem

      Volume: 411 Pages: 173-180

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ショウジョウバエを用いたマイオカインの生理的機能スクリーニング: Peroxiredoxin-6のマイオカインとしての生理的重要性の検討2017

    • Author(s)
      眞鍋康子, 武藤敬正, 山田健一朗, 片倉健悟, 青木友那, 古市泰郎, 坂井貴臣, 藤井宣晴
    • Organizer
      第5回骨格筋生物学研究会
    • Place of Presentation
      東京
    • Year and Date
      2017-03-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] 筋収縮をトリガーとしたマイオカイン分泌機序の解明2017

    • Author(s)
      古市泰郎, 眞鍋康子,宮田楓, 佐藤帆浪,高木麻由美,青木美穂,藤井宣晴
    • Organizer
      第5回骨格筋生物学研究会
    • Place of Presentation
      東京
    • Year and Date
      2017-03-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] Discovery of muscle contraction regulated myokine secretion in C2C12 myotubes2016

    • Author(s)
      Fujii NL, Manabe Y, Furuichi Y
    • Organizer
      第6回国際医科学ネットワークフォーラム
    • Place of Presentation
      松本
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] Peroxiredoxin-6のマイオカインとしての生理的重要性の検討2016

    • Author(s)
      眞鍋康子, 武藤敬正, 山田健一朗, 片倉健悟, 青木友那, 古市泰郎, 坂井貴臣, 藤井宣晴
    • Organizer
      第4回若手による骨格筋細胞研究会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] 急性筋収縮によるマイオカイン分泌機序の解明2016

    • Author(s)
      古市泰郎, 眞鍋康子,宮田楓, 佐藤帆浪,高木麻由美,青木美穂,藤井宣晴
    • Organizer
      第4回若手による骨格筋細胞研究会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-11-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規骨格筋発現遺伝子R3hdmlの機能解析2016

    • Author(s)
      坂本憲一, 竹本 稔, 古市泰郎, 眞鍋康子, 石川崇広, 前澤善朗, 清水孝彦, 藤井宣晴, 横手幸太郎
    • Organizer
      第31回日本糖尿病合併症学会
    • Place of Presentation
      仙台
    • Year and Date
      2016-10-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規マイオカインPeroxiredoxin6の分泌様式と生理的意義の検証2016

    • Author(s)
      眞鍋康子, 武藤敬正, 山田健一朗, 片倉健悟, 青木友那, 古市泰郎, 坂井貴臣, 藤井宣晴
    • Organizer
      第71回日本体力医学会大会
    • Place of Presentation
      盛岡
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] IL-15は急性筋収縮によって分泌促進されるマイオカインである2016

    • Author(s)
      古市泰郎,眞鍋康子,宮田楓, 佐藤帆浪, 高木麻由美, 青木美穂,藤井宣晴
    • Organizer
      第71回日本体力医学会大会
    • Place of Presentation
      盛岡
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Secretion of Myokines Regulated by Contraction in C2C12 myotube2016

    • Author(s)
      藤井宣晴
    • Organizer
      第48回日本動脈硬化学会(シンポジウム)
    • Place of Presentation
      東京
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 筋収縮に応じたマイオカイン分泌の証明 - バイオマーカーとしての可能性 -2016

    • Author(s)
      藤井宣晴
    • Organizer
      第5回TOBIRA研究交流フォーラム(主催者講演)
    • Place of Presentation
      東京
    • Year and Date
      2016-05-23
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 筋萎縮および筋肥大研究のための骨格筋培養細胞モデルの創出,2015

    • Author(s)
      藤井宣晴
    • Organizer
      日本分子生物学会 ワークショップ
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-02
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] An in vitro contraction model in mouse primary cultured myotubes using satellite cells originated from EDL and soleus2015

    • Author(s)
      Manabe Y, Ogino S., Ito M., Furuichi Y., Takagi M.,Yamada M., Goto-Inoue N., Ono Y.,. Fujii N.
    • Organizer
      International Academy of Sportology
    • Place of Presentation
      東京
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 糖代謝調節に関わる筋収縮調節性マイオカインの探索ストラテジー2015

    • Author(s)
      藤井宣晴
    • Organizer
      日本糖尿病学会 シンポジウム
    • Place of Presentation
      下関
    • Year and Date
      2015-05-22
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] 杏林書院2016

    • Author(s)
      藤井宣晴
    • Total Pages
      296
    • Publisher
      運動生理学から身体不活動の生理・分子生物学へ
    • Related Report
      2016 Annual Research Report
  • [Book] Springer2016

    • Author(s)
      Fujii NL
    • Total Pages
      257
    • Publisher
      Musculoskeletal Disease Associated With Diabetes Mellitus
    • Related Report
      2016 Annual Research Report
  • [Remarks] 運動分子生物学研究室 研究概要

    • URL

      http://www.comp.tmu.ac.jp/muscle/Kenkyuugaiyou.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 運動分子生物学

    • URL

      http://www.comp.tmu.ac.jp/muscle/kennkyuugyouseki.html

    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 筋張力測定システム及び筋張力測定方法2016

    • Inventor(s)
      眞鍋康子, 古市泰郎, 藤井宣晴
    • Industrial Property Rights Holder
      眞鍋康子, 古市泰郎, 藤井宣晴
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-06-23
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 骨格筋初代細胞の培養液2016

    • Inventor(s)
      古市泰郎, 眞鍋康子, 藤井宣晴
    • Industrial Property Rights Holder
      古市泰郎, 眞鍋康子, 藤井宣晴
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-06-23
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2022-06-09  

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