| Project/Area Number |
15K12669
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Sports science
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
Kamei Yasutomi 京都府立大学, 生命環境科学研究科, 教授 (70300829)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | エピジェネティクス / 骨格筋 / DNAメチル化 / サテライト細胞 / 遺伝子発現 |
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| Outline of Final Research Achievements |
In this study, we examined the involvement of epigenetic regulation (DNA methylation) in the determination of the constitution and metabolism of skeletal muscles. The overexpression of a transcriptional regulator PGC1a in skeletal muscles, typically observed following a long period of time-exercise, causes red fiber formation. Microarray analysis of the change in gene expression showed that PGC1a regulates the TCA cycle, oxidative phosphorylation, and fatty acid and branched-chain amino acid metabolism. We established a genome-wide DNA methylation analysis based on the combination of immunoprecipitation by a methyl-cytosine antibody and detection using a promoter genome array, which we originally designed. PGC1a overexpression in the skeletal muscle showed DNA methylation-mediated change in multiple gene promoters, possibly caused by red fiber formation.
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