Epigenetic regulation of skeletal muscle function via DNA methylation-mediated gene expression change
Project/Area Number |
15K12669
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Sports science
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Research Institution | Kyoto Prefectural University |
Principal Investigator |
Kamei Yasutomi 京都府立大学, 生命環境科学研究科, 教授 (70300829)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | エピジェネティクス / 骨格筋 / DNAメチル化 / サテライト細胞 / 遺伝子発現 |
Outline of Final Research Achievements |
In this study, we examined the involvement of epigenetic regulation (DNA methylation) in the determination of the constitution and metabolism of skeletal muscles. The overexpression of a transcriptional regulator PGC1a in skeletal muscles, typically observed following a long period of time-exercise, causes red fiber formation. Microarray analysis of the change in gene expression showed that PGC1a regulates the TCA cycle, oxidative phosphorylation, and fatty acid and branched-chain amino acid metabolism. We established a genome-wide DNA methylation analysis based on the combination of immunoprecipitation by a methyl-cytosine antibody and detection using a promoter genome array, which we originally designed. PGC1a overexpression in the skeletal muscle showed DNA methylation-mediated change in multiple gene promoters, possibly caused by red fiber formation.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition.2017
Author(s)
Ishikawa T, Kitaura Y, Kadota Y, Morishita Y, Ota M, Yamanaka F, Xu M, Ikawa M, Inoue N, Kawano F, Nakai N, Murakami T, Miura S, Hatazawa Y, Kamei Y, Shimomura Y.
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Journal Title
Sci Rep.
Volume: 7
Issue: 1
Pages: 39825-39825
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Down Regulation of Asparagine Synthetase and 3-Phosphoglycerate Dehydrogenase, and the Up-Regulation of Serine Dehydratase in Rat Liver from Intake of Excess Amount of Leucine Are Not Related to Leucine-Caused Amino Acid Imbalance2015
Author(s)
Yoshimura, R., Takai, M., Namaki, H., Minami, K., Imamura, W., Kato, H., Kamei, Y., and Kanamoto, R.
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Journal Title
Journal of Nutritional Science and Vitaminology
Volume: 61
Issue: 6
Pages: 441-448
DOI
NAID
ISSN
0301-4800, 1881-7742
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] PGC-1α-mediated changes in phospholipid profiles of exercise-trained skeletal muscle.2015
Author(s)
Senoo N, Miyoshi N, Goto-Inoue N, Minami K, Yoshimura R, Morita A, Sawada N, Matsuda J, Ogawa Y, Setou M, Kamei Y, Miura S.
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Journal Title
J Lipid Res.
Volume: 56
Issue: 12
Pages: 2286-96
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] ATM regulates adipocyte differentiation and contributes to glucose homeostasis.2015
Author(s)
Takagi M., Uno M., Nishii R., Sugimoto M., Hasegawa S., Piao J., Ihara N., Kanai S., Kakei S., Tamura Y., Suganami T., Kamei Y., Shimizu T., Yasuda A., Ogawa Y., Mizutani S.
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Journal Title
Cell Reports
Volume: 10
Issue: 6
Pages: 957-967
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ligand-activated PPARα-dependent DNA demethylation regulates the fatty acid β-oxidation genes in the postnatal liver.2014
Author(s)
Ehara T, Kamei Y, Yuan X, Takahashi M, Kanai S, Tamura E, Tsujimoto K, Tamiya T, Nakagawa Y, Shimano H, Takai-Igarashi T, Hatada I, Suganami T, Hashimoto K, Ogawa Y.
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Journal Title
Diabetes.
Volume: 64(3)
Issue: 3
Pages: 775-84
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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