Paradigm shift of the mechanisms that development of higher brain function on maternofetal transmission

• Project/Area Number: 15K12728
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Childhood science (childhood environment science)
• Research Institution: Tokai University
• Principal Investigator: TATEMICHI Masayuki 東海大学, 医学部, 教授 (00318263)
• Co-Investigator(Kenkyū-buntansha): 遠藤 整 東海大学, 医学部, 講師 (10550551)
• Research Collaborator: ETO Tomoo ; OWADA Satoshi
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 母体環境 / THAラット / 学習 / 次世代影響 / 高学習能 / 母子間伝達 / 子宮内環境

Outline of Final Research Achievements

While embryos transfer to recipient females is a useful experimental technique for biology and experimental animal research, it has yet to be determined whether these artificial reproduction technique and the maternal factor affect the phenotype of offspring, especially higher brain function. We showed that high learning ability of Tokai high avoider (THA) rat offspring was not replicate by embryos transfer technique utilizing surrogate female Wistar rats that are low avoidance phenotype. However, a postnatal cross-fostering investigation with the offspring of Wistar rat and THA rat showed that maternal care such as postnatal behavior and lactation trait were not much difference between dams of low avoidance Wistar rat and THA rat. We conclude that the offspring phenotype, although unchanged, has an imperceptible effect on the learning ability of embryos transfer-derived THA rats through the intrauterine environment of the recipient.

Research Products

• [Journal Article] The intrauterine environment affects learning ability of Tokai high avoider rat offspring derived using cryopreservation and embryo transfer-mediated reproduction. (2017)
  • Author(s): Endo H, Eto T, Yoshii F, Owada S, Watanabe T, Tatemichi M, Kimura M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 22;489(2) Issue: 2 Pages: 211-216
  • DOI: 10.1016/j.bbrc.2017.05.133
  • Peer Reviewed
• [Journal Article] Acetamiprid Accumulates in Different Amounts in Murine Brain Regions. (2016)
  • Author(s): Terayama H, Endo H, Tsukamoto H, Matsumoto K, Umezu M, Kanazawa T, Ito M, Sato T, Naito M, Kawakami S, Fujino Y, Tatemichi M, Sakabe K.
  • Journal Title: Int J Environ Res Public Health Volume: 13(10) Issue: 10 Pages: 937-937
  • DOI: 10.3390/ijerph13100937
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Remarks] 東海大学医学部基盤診療学系 衛生学公衆衛生学
  • URL: http://health.med.u-tokai.ac.jp/index.html
• [Remarks] 東海大学医学部衛生学公衆衛生学
  • URL: http://health.med.u-tokai.ac.jp/research.html