Construction of convergent library for fungal cytochrome P450s and its application to exploring novel functions
Project/Area Number |
15K12737
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Biomolecular chemistry
|
Research Institution | Hokkaido University |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
MINAMI Atsushi 北海道大学, 大学院理学研究院, 助教 (40507191)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | チトクロームP450 / 酸化酵素 / 天然物 / 生合成 |
Outline of Final Research Achievements |
Heterologous expression of cytochrome P450 for fungal natural product biosynthesis has been employed. We succeeded to prepare library of multiple P450-expressed transformants. Using this unique library, we performed the screening the transformants and found various conversion to generate a number of new metabolites although their productions were relatively low.
|
Report
(3 results)
Research Products
(5 results)
-
-
-
[Presentation] 抗生物質プロイロムチリンの生合成研究 (3)2017
Author(s)
山根 桃華,南 篤志,劉 成偉,尾崎 太郎,塚越 多映,常盤野 哲生,五味 勝也,及川 英秋
Organizer
日本化学会 第97春季年会 2017
Place of Presentation
慶應義塾大学 日吉キャンパス(神奈川県・横浜市)
Year and Date
2017-03-16
Related Report
-
-