Development of a new method for identifying substrates of PPM1 phosphatase family

• Project/Area Number: 15K12738
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Hokkaido University
• Principal Investigator: Sakaguchi Kazuyasu 北海道大学, 理学研究院, 教授 (00315053)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 酵素 / 脱リン酸化 / プロテインホスファターゼ / 基質 / 蛋白質 / 翻訳後修飾 / ホスファターゼ / ペプチドライブラリー

Outline of Final Research Achievements

Protein Phosphorylation and dephosphorylation is a key mechanism for regulation of protein functions. Human PPM1 phosphatase family is composed of 12 members that play important roles in wide variety of cellular responses, such as differentiation, cell cycle regulation, and metabolism. However, there is not a versatile method to find cellular targets and substrates for these phosphatases. In this study, we designed a new method for identifying substrates and demonstrated that this method is very effective.

Research Products

• [Presentation] 多様なシグナル経路に関与するPPM1Aホスファターゼの脱リン酸化部位認識機構 (2016)
  • Author(s): 白幡祐貴子、清田雄平、松山祐昂、西村裕一、中馬吉郎、下東康幸、坂口和靖
  • Organizer: 日本化学会秋季事業第6回CSJフェスタ2016
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2016-11-14
• [Presentation] Regulation of adipocyte differentiation and lipid droplet formation by PPM1D phosphatase (2016)
  • Author(s): Kazuyasu Sakaguchi
  • Organizer: 第12回プロテインホスファターゼ国際カンファレンス
  • Place of Presentation: 近畿大学東大阪キャンパス（大阪府・東大阪市）
  • Year and Date: 2016-10-27
  • Int'l Joint Research / Invited
• [Presentation] Ser/ThrホスファターゼPPM1A（PP2C-a）の脱リン酸化部位認識機構 (2016)
  • Author(s): 白幡祐貴子、清田雄平、松山祐昂、鎌田瑠泉、下東康幸、坂口和靖
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2016-09-25
• [Presentation] p53-inducible phosphatase PPM1D: Function, regulation and inhibitor deveolment (2016)
  • Author(s): Kazuyasu Sakaguchi
  • Organizer: 16th Akabori Conference
  • Place of Presentation: 六甲山ホテル（兵庫県・神戸市）
  • Year and Date: 2016-05-23
  • Int'l Joint Research / Invited
• [Presentation] 脂肪細胞におけるp53誘導性ホスファターゼPPM1D阻害剤の 脂肪滴形成抑制効果 (2016)
  • Author(s): 鎌田瑠泉、木村望、小笠原紗里、工藤風樹、清田雄平、坂口和靖
  • Organizer: 第７回プロテインホスファターゼ研究会
  • Place of Presentation: 基礎生物学研究所（愛知県・岡崎市）
  • Year and Date: 2016-01-29
  • Invited
• [Presentation] リン脂質のPPM1ホスファターゼILKAP脱リン酸化活性に対する効果 (2016)
  • Author(s): 塚原七星,清田雄平,白幡祐貴子,鎌田瑠泉,坂口和靖
  • Organizer: 第７回プロテインホスファターゼ研究会
  • Place of Presentation: 基礎生物学研究所（愛知県・岡崎市）
  • Year and Date: 2016-01-29
• [Presentation] Tetramerization Domain of Tumor Suppressor Protein p53: Evolution, Mutation and Application (2015)
  • Author(s): Kazuyasu Sakaguchi
  • Organizer: 7th International Peptide Symposium
  • Place of Presentation: Biopolis (Singapore)
  • Year and Date: 2015-12-09
  • Int'l Joint Research / Invited
• [Presentation] Oncogenic phosphatase PPM1D: Role in carcinogenesis and its inhibitors (2015)
  • Author(s): 坂口和靖
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートピアホテル（兵庫県・神戸市）
  • Year and Date: 2015-12-04
  • Invited
• [Presentation] PPM1ホスファターゼILKAPの脱リン酸化能に対するリ ン脂質の効果 (2015)
  • Author(s): 塚原七星、清田雄平、白幡祐貴子、鎌田瑠泉、坂口和靖
  • Organizer: 第５回CSJフェスタ2015
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2015-10-15
• [Presentation] PPM1Dホスファターゼ阻害剤の急性前骨髄球性白血病細胞株HL-60分化に対する効果 (2015)
  • Author(s): 工藤風樹、鎌田瑠泉、吉村文彦、谷野圭持、坂口和靖
  • Organizer: 第52回日本生化学会北海道支部例会
  • Place of Presentation: 北海道大学（北海道・札幌市
  • Year and Date: 2015-07-17