| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Outline of Final Research Achievements |
We developed the protein modification technique which take place in the proximity of a few nanometers around the ruthenium photocatalyst. The two types of small molecules, the Ru / dcbpy complex and the ligand molecule of the target protein, were modified on the beads. This ruthenium photocatalyst-functionalized affinity beads enable the target selective purification from protein mixture and chemical labeling simultaneously without significant non-specific binding proteins to the beads. In addition, (1) the high sensitive detection of ligand binding protein with chemical labeling, (2) the detection of low affinity ligand binding protein that is difficult to be detected by conventional affinity chromatography method, (3) antibody purification and chemical labeling were achieved.
|
