| Project/Area Number |
15K12745
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomolecular chemistry
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
Shiroh Futaki 京都大学, 化学研究所, 教授 (50199402)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | イオンチャネル / アプタマー / アラメチシン / センサー / DNAアプタマー |
|---|
| Outline of Final Research Achievements |
In this research, we aimed to construct an alamethicin channel with a nucleic acid aptamer which changes its conformation by binding with a ligand in the extramembrane region, with a view to developing into a tailored sensor capable of responding to various ligands. Preparation of alamethicin-nucleic acid hybrids was conducted by click reaction. Although this hybrid molecule formed ion channels in the lipid bilayer, it was found that the hybridized alamethicin destabilizes the lipid membrane and it was difficult to obtain reproducible channel recordings. In addition, the change in the channel current due to the structural alteration of the nucleic acid segment was not conspicuous, and it was found that it is necessary to reexamine the structural design in the future.
|
