| Project/Area Number |
15K12755
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
Kawai Kiyohiko 大阪大学, 産業科学研究所, 准教授 (50314422)
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| Co-Investigator(Renkei-kenkyūsha) |
MARUYAMA Atsushi 東京工業大学, 生命理工学院, 教授 (40190566)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | DNA / supercoil / fluorescence / blinking / 1分子測定 / 超らせん / 1分子計測 / 三本鎖 / 蛍光相関分光 / 速度 / DNA高次構造 / 蛍光 / 3重鎖 |
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| Outline of Final Research Achievements |
In our body, DNA takes a negatively-supercoiled structure. It is suggested that negative-supercoil induces strand dissociation that leads to the formation of triple helix DNA. In this project, in order to investigate the dynamics of negatively-supercoiled DNA, we developed a method to detect the formation of the DNA triple helix structure at the single molecule level.
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