| Project/Area Number |
15K12756
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 進化分子工学 / スクリーニング / 膜タンパク質 / ペプチド / 無細胞翻訳系 |
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| Outline of Final Research Achievements |
We aim to construct an in vitro screening system for peptides that inhibit the function of membrane protein of interest. The method is based on the syntheses of membrane protein of interest and the inhibitor peptide simultaneously using an in vitro transcription-translation system inside cell-sized phospholipid vesicles. Here, we first created a high quality random peptide library with a length of 10 amino acids. Then using EmrE, a multidrug transporter from Escherichia coli as a model protein, the screening system of an inhibitor peptide was established. By combining the constructed peptide library and the gene screening system, we now can screen inhibitor peptides entirely in vitro.
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