Challenge towards single-cell MS imaging

• Project/Area Number: 15K12762
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Takahashi Katsutoshi 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (00271792)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 質量イメージング / 単一細胞分析 / MALDI / FTICR-MS / 単一細胞 / 紫外パルスレーザー / マトリクス / 植物組織

Outline of Final Research Achievements

The laser focus optics of current in-house built MALDI-FTICRMS was improved so as to decrease the laser focus spot size onto the specimen to less than 5 micron from 10 micron.

The laser optics was also modified to enable the on-axis irradiation of different wavelength laser light with precisly controlled different timing to burn through-hole on the non-conductive thick sample specimen to prevent charging-up on the sample specimen during MS imaing measurement.

Research Products

• [Journal Article] Spatially resolved metabolic distribution for unraveling the physiological change and responses in tomato fruit using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) (2017)
  • Author(s): Nakamura J, Morikawa-Ichinose T, Fujimura Y, Hayakawa E, Takahashi K, Ishii T, Miura D, and Wariishi H
  • Journal Title: Anal Bioanal Chem Volume: 409 Issue: 6 Pages: 1697-1706
  • DOI: 10.1007/s00216-016-0118-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Spatial variations in the molecular diversity of dissolved organic matter in water moving through a boreal forest in eastern Finland. (2017)
  • Author(s): Jun’ichiro Ide, Mizue Ohashi, Katsutoshi Takahashi, Yuko Sugiyama, Sirpa Piirainen, Pirkko Kortelainen, Nobuhide Fujitake, Keitaro Yamase, Nobuhito Ohte, Mina Moritani, Miyako Hara, Leena Finer
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 42102-42102
  • DOI: 10.1038/srep42102
  • NAID: 120006360176
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 2)Cell-specific localization of alkaloids in Catharantus roseus stem tissue measured with Imaging MS and Single cell MS (2016)
  • Author(s): K.Yamamoto, K.Takahashi, H.Mizuno, A.Anegawa, K.Ishizaki, H.Fukaki, M.Ohnishi, M.Yamazaki, T.Masujima, T.Mimura
  • Journal Title: Proceeding of the National Academy of Sciences of the United States of America Volume: 113 Issue: 14 Pages: 3891-3896
  • DOI: 10.1073/pnas.1521959113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 1)Development and application of a high resolution imaging mass spectroscope for the study of plant tissues (2015)
  • Author(s): K.Takahashi, T.Kozuka, A.Anegawa, A.Nagatani, T.Mimura
  • Journal Title: PLANT AND CELL PHYSIOLOGY Volume: 56 Issue: 7 Pages: 1329-1338
  • DOI: 10.1093/pcp/pcv083
  • Peer Reviewed / Acknowledgement Compliant