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Challenge towards single-cell MS imaging

Research Project

Project/Area Number 15K12762
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Takahashi Katsutoshi  国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (00271792)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords質量イメージング / 単一細胞分析 / MALDI / FTICR-MS / 単一細胞 / 紫外パルスレーザー / マトリクス / 植物組織
Outline of Final Research Achievements

The laser focus optics of current in-house built MALDI-FTICRMS was improved so as to decrease the laser focus spot size onto the specimen to less than 5 micron from 10 micron.

The laser optics was also modified to enable the on-axis irradiation of different wavelength laser light with precisly controlled different timing to burn through-hole on the non-conductive thick sample specimen to prevent charging-up on the sample specimen during MS imaing measurement.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results)

  • [Journal Article] Spatially resolved metabolic distribution for unraveling the physiological change and responses in tomato fruit using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI)2017

    • Author(s)
      Nakamura J, Morikawa-Ichinose T, Fujimura Y, Hayakawa E, Takahashi K, Ishii T, Miura D, and Wariishi H
    • Journal Title

      Anal Bioanal Chem

      Volume: 409 Issue: 6 Pages: 1697-1706

    • DOI

      10.1007/s00216-016-0118-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Spatial variations in the molecular diversity of dissolved organic matter in water moving through a boreal forest in eastern Finland.2017

    • Author(s)
      Jun’ichiro Ide, Mizue Ohashi, Katsutoshi Takahashi, Yuko Sugiyama, Sirpa Piirainen, Pirkko Kortelainen, Nobuhide Fujitake, Keitaro Yamase, Nobuhito Ohte, Mina Moritani, Miyako Hara, Leena Finer
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 42102-42102

    • DOI

      10.1038/srep42102

    • NAID

      120006360176

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 2)Cell-specific localization of alkaloids in Catharantus roseus stem tissue measured with Imaging MS and Single cell MS2016

    • Author(s)
      K.Yamamoto, K.Takahashi, H.Mizuno, A.Anegawa, K.Ishizaki, H.Fukaki, M.Ohnishi, M.Yamazaki, T.Masujima, T.Mimura
    • Journal Title

      Proceeding of the National Academy of Sciences of the United States of America

      Volume: 113 Issue: 14 Pages: 3891-3896

    • DOI

      10.1073/pnas.1521959113

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 1)Development and application of a high resolution imaging mass spectroscope for the study of plant tissues2015

    • Author(s)
      K.Takahashi, T.Kozuka, A.Anegawa, A.Nagatani, T.Mimura
    • Journal Title

      PLANT AND CELL PHYSIOLOGY

      Volume: 56 Issue: 7 Pages: 1329-1338

    • DOI

      10.1093/pcp/pcv083

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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