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Branch analysis of biopolymers based on voltage-controlled single molecule translocation across a solid-state nanopore

Research Project

Project/Area Number 15K13294
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Nanomaterials chemistry
Research InstitutionTokyo Denki University (2017)
Institute of Physical and Chemical Research (2015-2016)

Principal Investigator

TAKEMASA Makoto  東京電機大学, 理工学部, 准教授 (30318795)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsナノポア / 多糖類 / 分岐 / FPGA / 1分子 / 多糖 / 分岐高分子
Outline of Final Research Achievements

Sequential analysis of single molecule was performed using a solid-state nanopore, a small hole fabricated on thin membrane. Driving force of the translocation across the membrane is mainly an electrophoretic force based on the voltage applied across the membrane. By changing this voltage level and/or sign just after the entrance of each molecule into the nanopore, we tried to control the speed and/or direction of the translocation of the molecule.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] 固体ナノポアを通過する方向及び速度制御による高分子の1分子分析2017

    • Author(s)
      武政誠, 藤田雅弘, 前田瑞夫
    • Organizer
      第66回高分子討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ナノポアによる高分子の1分子解析2017

    • Author(s)
      武政誠, 藤田雅弘, 前田瑞夫
    • Organizer
      信州コロイド・界面科学研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Control of translocation speed toward more accurate single molecule polymer analysis2016

    • Author(s)
      Makoto Takemasa, Masahiro Fujita, Mizuo Maeda
    • Organizer
      第65回高分子討論会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 固体ナノポアによるバイオ高分子の1分子解析2016

    • Author(s)
      武政 誠、藤田 雅弘、前田 瑞夫
    • Organizer
      第26回バイオ・高分子シンポジウム
    • Place of Presentation
      東京工業大学
    • Year and Date
      2016-07-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Single molecule analysis of polysaccharides using a solid state nanopore2015

    • Author(s)
      Makoto Takemasa, Masahiro Fujita, Mizuo Maeda
    • Organizer
      Pacifichem
    • Place of Presentation
      Marriott Waikiki Beach, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Single molecular analysis of biomolecules using a solid state nanopore2015

    • Author(s)
      Makoto Takemasa, Masahiro Fujita, Mizuo Maeda
    • Organizer
      8th KIFEE symposium
    • Place of Presentation
      NTNU, Trondheim, Norway
    • Year and Date
      2015-09-23
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ナノポア式1分子構造解析法の高精度化に向けた分子の移動速度制御2015

    • Author(s)
      武政 誠、藤田 雅弘、前田 瑞夫
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      東北大学
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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