| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
It is expected to develop a technique for artificially designing and arranging various proteins responsible for biochemical reactions and reconstructing the biochemical reaction network.In this study, we propose a method to create peptide ligase that enables intermolecular coupling of proteins and peptides, and accumulation on solid phase, and developed and verified necessary basic technologies. We developed a technique to immobilize the cell-free synthesized protein on the solid phase using sortase, a protein transferase derived from gram-positive bacteria. Then we experimentally clarified the problems which become prominent in the coexistence of cell-free translation and sortase reaction system, and discussed their solutions.These results are expected to contribute to the future development of screening technology for sortase variants by combining with microarray chip technology.
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