Desing method of intracellular RNA-protein structure
| Project/Area Number |
15K13314
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanobioscience
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| Research Institution | Kyoto University |
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Principal Investigator |
Fujita Yoshihiko 京都大学, iPS細胞研究所, 特定拠点助教 (60589266)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | RNA / synthetic biology / nano technology / nanotechnology / RNA switch / miRNA |
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| Outline of Final Research Achievements |
We succeeded in controlling the cellular function depending on the intracellular environment by designed artificial RNA that interacts with endogenous protein and forms RNA-protein nanostructures. We also succeeded in constructing an RNA switch whose translational level increased in response to the activity of intracellular miRNA. Both are fundamental technologies for controlling the cellular functions. Notably, designed miRNA-responsive RNA switches enabled us to remove specific cells without a cell sorter. This result indicates the possibility of medical applications such as removal of cancer cells and undifferentiated.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.2017
Author(s)
Tomonori Shibata, Yoshihiko Fujita, Hirohisa Ohno, Yuki Suzuki, Karin Hayashi, Kaoru R. Komatsu, Shunsuke Kawasaki, Kumi Hidaka, Shin Yonehara, Hiroshi Sugiyama, Masayuki Endo and Hirohide Saito
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Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 540-540
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] iPS細胞中で働くセンサー2016
Author(s)
藤田祥彦、齊藤博英
Organizer
新学術領域「分子ロボティクス」第7回領域会議
Place of Presentation
西浦温泉ホテルたつき(愛知県蒲郡市)
Year and Date
2016-03-14
Related Report
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[Book] 細胞工学2015
Author(s)
藤田 祥彦, 齊藤 博英
Total Pages
7
Publisher
学研メディカル秀潤社
Related Report
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