Desing method of intracellular RNA-protein structure

• Project/Area Number: 15K13314
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: Fujita Yoshihiko 京都大学, iPS細胞研究所, 特定拠点助教 (60589266)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: RNA / synthetic biology / nano technology / nanotechnology / RNA switch / miRNA

Outline of Final Research Achievements

We succeeded in controlling the cellular function depending on the intracellular environment by designed artificial RNA that interacts with endogenous protein and forms RNA-protein nanostructures. We also succeeded in constructing an RNA switch whose translational level increased in response to the activity of intracellular miRNA. Both are fundamental technologies for controlling the cellular functions. Notably, designed miRNA-responsive RNA switches enabled us to remove specific cells without a cell sorter. This result indicates the possibility of medical applications such as removal of cancer cells and undifferentiated.

Research Products

• [Int'l Joint Research] University of Seoul(韓国)
• [Journal Article] Competition between translation initiation factor eIF5 and its mimic protein 5MP determines non-AUG initiation rate genome-wide (2017)
  • Author(s): Tang Leiming、Morris Jacob、Wan Ji、Moore Chelsea、Fujita Yoshihiko、Gillaspie Sarah、Aube Eric、Nanda Jagpreet、Marques Maud、Jangal Maika、Anderson Abbey、Cox Christian、Hiraishi Hiroyuki、Dong Leiming、Saito Hirohide、Singh Chingakham Ranjit、Witcher Michael、Topisirovic Ivan、Qian Shu-Bing、Asano Katsura
  • Journal Title: Nucleic Acids Research Volume: 45 Issue: 20 Pages: 11941-11953
  • DOI: 10.1093/nar/gkx808
  • NAID: 120006537139
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate. (2017)
  • Author(s): Tomonori Shibata, Yoshihiko Fujita, Hirohisa Ohno, Yuki Suzuki, Karin Hayashi, Kaoru R. Komatsu, Shunsuke Kawasaki, Kumi Hidaka, Shin Yonehara, Hiroshi Sugiyama, Masayuki Endo and Hirohide Saito
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 540-540
  • DOI: 10.1038/s41467-017-00459-x
  • NAID: 120006346264
  • Peer Reviewed / Open Access
• [Journal Article] Cell-type-specific genome editing with a microRNA-responsive CRISPR?Cas9 switch (2017)
  • Author(s): Hirosawa Moe、Fujita Yoshihiko、Parr Callum、Hayashi Karin、Kashida Shunnichi、Hotta Akitsu、Woltjen Knut、Saito Hirohide
  • Journal Title: Nucleic Acids Research Volume: 45 Issue: 13 Pages: e118-e118
  • DOI: 10.1093/nar/gkx309
  • NAID: 120006306001
  • Peer Reviewed / Open Access
• [Journal Article] Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells (2017)
  • Author(s): Kawasaki Shunsuke、Fujita Yoshihiko、Nagaike Takashi、Tomita Kozo、Saito Hirohide
  • Journal Title: Nucleic Acids Research Volume: 45 Issue: 12 Pages: e117-e117
  • DOI: 10.1093/nar/gkx298
  • NAID: 120006305984
  • Peer Reviewed / Open Access
• [Journal Article] RNA-based gene circuits for cell regulation (2016)
  • Author(s): Karagiannis P, Fujita Y, Saito H*.
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 92 Issue: 9 Pages: 412-422
  • DOI: 10.2183/pjab.92.412
  • NAID: 130005277956
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Safe and versatile miRNA-responsive ON switch (2017)
  • Author(s): Yoshihiko Fujita, Karin Hayashi, Hirohide Saito
  • Organizer: THE 43rd NAITO CONFERENCE ON Noncoding RNA : Biology, Chemistry, & Diseases
  • Int'l Joint Research
• [Presentation] Construction of Simple miRNA responsive ON switch (2017)
  • Author(s): Yoshihiko Fujita, Hirohide Saito
  • Organizer: 第１9回日本RNA学会年会
• [Presentation] RNA合成生物学からの再生医療へのアプローチ (2017)
  • Author(s): 藤田 祥彦
  • Organizer: 学長裁量経費プロジェクト 「理工医薬RNAアライアンス」主催 富山RNAワークショップ
  • Invited
• [Presentation] RNA/RNPによる医療応用へ向けたDNAフリーな遺伝子回路 (2017)
  • Author(s): 藤田 祥彦
  • Organizer: ConBio2017
• [Presentation] miRNA-responsive ON switch using non-canonical mRNA structure (2017)
  • Author(s): Yoshihiko Fujita, Karin Hayashi, Hirohide Saito
  • Organizer: ISBC2017
  • Int'l Joint Research
• [Presentation] RNA合成生物学からの再生医療へのアプローチ (2017)
  • Author(s): 藤田 祥彦
  • Organizer: 富山RNAワークショップ
  • Place of Presentation: 富山大学 杉谷キャンパス
  • Invited
• [Presentation] Design of a miRNA-responsive ON switch (2016)
  • Author(s): Yoshihiko Fujita, Karin Hayashi, Hirohide Saito
  • Organizer: RNA 2016
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] iPS細胞中で働くセンサー (2016)
  • Author(s): 藤田祥彦、齊藤博英
  • Organizer: 新学術領域「分子ロボティクス」第7回領域会議
  • Place of Presentation: 西浦温泉ホテルたつき(愛知県蒲郡市)
  • Year and Date: 2016-03-14
• [Presentation] miRNA応答mRNAの設計と細胞のmiRNA活性プロファイルの作成 (2016)
  • Author(s): 藤田 祥彦, 齊藤博英
  • Organizer: 分子ロボティクス研究会
  • Place of Presentation: 京都大学物質-細胞統合システム拠点 本館 2 階セミナー室 京都市左京区吉田本町
  • Year and Date: 2016-02-20
  • Invited
• [Presentation] 人工RNAシステムを利用した遺伝子操作と細胞運命制御 (2015)
  • Author(s): 藤田 祥彦, 齊藤博英
  • Organizer: 日本進化学会
  • Place of Presentation: 中央大学後楽園キャンパス 東京都文京区春日1-13-27
  • Year and Date: 2015-08-20
  • Invited
• [Presentation] RNP ナノ構造の空間配置を利用したレセプターの信号制御 (2015)
  • Author(s): 藤田 祥彦, 齊藤博英
  • Organizer: 2015 年 RNA学会
  • Place of Presentation: ホテルライフォート札幌 北海道札幌市中央区南10条西1-1-30
  • Year and Date: 2015-07-15
• [Book] 細胞工学 (2015)
  • Author(s): 藤田 祥彦, 齊藤 博英
  • Total Pages: 7
  • Publisher: 学研メディカル秀潤社
• [Patent(Industrial Property Rights)] 巨核球細胞群における細胞の不均質性を識別する方法 (2017)
  • Inventor(s): 江藤浩之/齊藤博英/橋本一哉/藤田祥 彦/他3名
  • Industrial Property Rights Holder: 江藤浩之/齊藤博英/橋本一哉/藤田祥 彦/他3名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Overseas
• [Patent(Industrial Property Rights)] miRNA 応答性マーカー遺伝子を有する mRNA ライブラリ作製法 (2017)
  • Inventor(s): 齊藤 博英/藤田 祥彦
  • Industrial Property Rights Holder: 齊藤 博英/藤田 祥彦
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-209406
  • Filing Date: 2017