| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We propose a packaging technology using DNA molecules as scaffolds for realizing high-density and precise arrangement of DNA nano-devices. Single DNA molecules are extended and immobilized between micro-electrodes to form "DNA highwires".
In this project, we focused on a DNA manipulation technique under a fluorescence microscope for forming DNA highwires. We successfully manipulated single DNA molecules using two microstructures driven by two laser beams, where we handled them like ‘chopsticks’. We evaluated the dimensions and the shapes of microstructures, intended for holding single DNA molecules between them efficiently. We have found that the geometry of the structure is dominant parameter for effective handling of single DNA molecules. This manipulation technique allows the imaging of single DNA molecules with its intact state, which will lead to fabrication of DNA nanosystems.
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