Study on interactions between nanovesicles and solid surfaces toward new exosome medicine
Project/Area Number |
15K13361
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Thin film/Surface and interfacial physical properties
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Research Institution | Yokohama National University |
Principal Investigator |
Ogino Toshio 横浜国立大学, 大学院工学研究院, 教授 (70361871)
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Co-Investigator(Renkei-kenkyūsha) |
Shiba Kiyotaka 公益財団法人がん研究会, がん研究所, 部長 (40196415)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | エクソソーム / ガン診断 / 固体表面 / 脂質二分子膜 / 吸着 / 機械学習 / 原子間力顕微鏡 / 固体基板 / 脂質二重膜 / タンパク質 |
Outline of Final Research Achievements |
Exosomes are nanovesicles with 30-150 nm in diameter and released from whole cells including cancer cells. In this study, we have developed a novel method to extract exosome properties using atomic force microscopy (AFM) for early diagnosis of cancer. When exosomes are immobilized on solid surfaces, they are deformed by interactions between biomolecules and sold surfaces. From this deformation, much information about exosome membranes and inclusions can be obtained. To precisely discriminate the deformation fashion of individual exosomes, we used machine learning, where 14 parameters are extracted from the individual AFM images and plotted in a 14-dimensional space. By plotting AFM data from unknown exosomes in the discriminator, we can assign those exosomes to the specific host cell. We have found that the correct assignment with probability from 65 to 85% can be achieved.
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Report
(3 results)
Research Products
(38 results)
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[Presentation] Applications of biointerface control to bioelectronics and nanomedicine2017
Author(s)
Toshio Ogino, Kazuki Ito, Naotoshi Sakaguchi, Tamiko Minamisawa, Sachiko Matsumura, Kanako Suga, Kiyotaka Shiba, Yasuo Kimura, Ayumi Hirano-Iwata
Organizer
The Int. Surfaces, Coatings and Interfaces Conference
Place of Presentation
Incheon, Korea
Year and Date
2017-03-29
Related Report
Int'l Joint Research / Invited
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[Presentation] Control of biointerfaces and its applications to exosome characterization2017
Author(s)
Toshio Ogino, Kazuki Ito, Yuta Ogawa, Masaki Nakamura, Tamiko Minamisawa, Sachiko Matsumura, Kanako Suga, Kiyotaka Shiba, Yasuo Kimura, Ayumi Hirano-Iwata
Organizer
Japan-Taiwan Nanomedicine
Place of Presentation
Taipei, Taiwan
Year and Date
2017-03-16
Related Report
Int'l Joint Research / Invited
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[Presentation] 放出元細胞の同定に向けたエクソソームの基板吸着様態を用いる特徴抽出2016
Author(s)
伊藤和希, 小川裕太, 横田圭司, 松村幸子, 南澤宝美后, 菅加奈子, 芝清隆, 木村康男, 平野愛弓, 荻野俊郎
Organizer
日本表面科学会, 2016年真空・表面科学合同講演会
Place of Presentation
名古屋
Year and Date
2016-11-29
Related Report
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[Presentation] Characterization of exosomes using atomic force microscopy and scanning electron microscopy2016
Author(s)
Toshio Ogino, Kazuki Ito, Keiji Yokota, Yuta Ogawa, Tamiko Minamisawa, Sachiko Matsumura, Kanako Suga, Kiyotaka Shiba, Yasuo Kimura, Ayumi Hirano-Iwata
Organizer
13th Int. Conf. on Atomically Controlled Surfaces, Interfaces and Nanostructures
Place of Presentation
Frascati, Italy
Year and Date
2016-10-09
Related Report
Int'l Joint Research / Invited
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[Presentation] Extraction of morphological features of exosomes adsorbed to solid substrates toward host cell identification2016
Author(s)
Kazuki Ito, Yuta Ogawa, Keiji Yokota, Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Kiyotaka Shiba, Yasuo Kimura, Ayumi Hirano-Iwata, Toshio Ogino
Organizer
20th Int. Vacuum Congress
Place of Presentation
Busan, Korea
Year and Date
2016-08-21
Related Report
Int'l Joint Research
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[Presentation] 固体基板への固定による生体物質の評価2016
Author(s)
荻野俊郎, 伊藤和希, 横田圭司, 小川裕太, 松村幸子, 南澤宝美后, 菅加奈子, 芝清隆, 木村康男, 平野愛弓
Organizer
電子情報通信学会・電子デバイス研究会
Place of Presentation
米沢
Year and Date
2016-04-21
Related Report
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[Presentation] ホスト細胞同定に向けたエクソソーム基板吸着様態の特徴抽出”, 応用物理学会春季学術講演会2016
Author(s)
伊藤和希, 小川裕太, 横田圭司, 松村幸子, 南澤宝美后, 菅加奈子, 芝清隆, 木村康男, 平野愛弓, 荻野俊郎
Organizer
応用物理学会春季学術講演会
Place of Presentation
東京
Year and Date
2016-03-20
Related Report
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[Presentation] Characterization of Exosomes using Atomic Force Microscopy and Scanning Electron Microscopy2016
Author(s)
Toshio Ogino, Kazuki Ito, Keiji Yokota, Yuta Ogawa, Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Kiyotaka Shiba, Yasuo Kimura, Ayumi Hirano-Iwata
Organizer
10th International Symposium on Medical, Bio- and Nano-Electronics
Place of Presentation
仙台
Year and Date
2016-03-03
Related Report
Int'l Joint Research
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