| Project/Area Number |
15K13379
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Optical engineering, Photon science
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| Research Institution | Yokohama National University |
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Principal Investigator |
Baba Toshihiko 横浜国立大学, 大学院工学研究院, 教授 (50202271)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Keywords | 細胞イメージング / GaInAsP / フォトルミネセンス / ラベルフリー |
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| Outline of Final Research Achievements |
In this study, we proposed and demonstrated a semiconductor imaging plate that images various cells without using fluorescent labels. This is based on the principle that the photoluminescence (PL) from GaInAsP semiconductor changes with pH and surface charge. When cancer cells, normal cells, stem cells and so on were adsorbed on the semiconductor imaging plate and the PL image was observed with an infrared camera, an image reflecting the cells was captured. It was also confirmed that they corresponded to fluorescence images, and that the extracellular matrix as the scaffolding material of the cell strongly relates with the images. We tried to distinguish between cancer cells and normal cells, but could not observe clear difference. Some optimization of culture solution and so on may be necessary to achieve this function.
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