Multi-frequency force modulation AFM: Mapping single cell rheological parameters

• Project/Area Number: 15K13393
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General applied physics
• Research Institution: Hokkaido University
• Principal Investigator: OKAJIMA TAKAHARU 北海道大学, 情報科学研究科, 教授 (70280998)
• Co-Investigator(Renkei-kenkyūsha): SUEOKA KAZUHISA 北海道大学, 情報科学研究科, 教授 (60250479)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 原子間力顕微鏡 / 細胞レオロジー

Outline of Final Research Achievements

In this study, I developed multi-frequency force modulation atomic force microscopy (AFM) which enable mapping rheological properties of living cells in their physiological conditions. The AFM method was applied to investigate (1) the relationship between cell rheology and cell cytoskeleton at the single cell level, (2) single cancer cell mechanical diagnostics, and (3) ensemble and temporal variations of single cell rheology.

Research Products

• [Journal Article] Comparison between power-law rheological parameters of living cells in frequency and time domains measured by atomic force microscopy (2016)
  • Author(s): R. Takahashi, T. Okajima
  • Journal Title: Japanese Journal of Applied Physics Volume: 55 Issue: 8S1 Pages: 08NB22-08NB22
  • DOI: 10.7567/jjap.55.08nb22
  • NAID: 210000146989
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy (2015)
  • Author(s): R. Takahashi, T. Okajima
  • Journal Title: Applied Physics Letters Volume: 107 Issue: 17 Pages: 173702-173702
  • DOI: 10.1063/1.4934874
  • NAID: 120005677755
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Diagnosing single cell diseases by SPM (2016)
  • Author(s): M. Sawano*, K. Aoki, R. Tanaka, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, N.J. Cho, T. Okajima
  • Organizer: 24th International Colloquium on Scanning Probe Microscopy (ICSPM24)
  • Place of Presentation: Hawaii(Hawaii Convention Center)
  • Year and Date: 2016-12-14
  • Int'l Joint Research
• [Presentation] Mapping cell-to-cell variations in power-law rheology investigated by multifrequency force modulation atomic force microscopy (2016)
  • Author(s): M. Sawano, R. Tanaka, R. Takahashi, K. Kurubayashi-Shigetomi, A. Subagyo, K. Sueoka, T.Okajima
  • Organizer: 2016 ASCB Annual Meeting
  • Place of Presentation: San Francisco（Moscone Center）
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Mapping elastic modulus of single cells and tissue in stable state and morphogenesis by atomic force microscopy (2016)
  • Author(s): Takaharu Okajima
  • Organizer: 4th Kanazawa Bio-AFM Workshop
  • Place of Presentation: Kanazawa（KKR Hotel Kanazawa）
  • Year and Date: 2016-10-03
  • Int'l Joint Research / Invited
• [Presentation] 原子間力顕微鏡による 1 細胞力学診断：細胞レオロジー特性の ばらつきの定量評価 (2016)
  • Author(s): 澤野麻紀*、田中良典、繁富（栗林）香織、スバギョ アグス、末岡和久、岡嶋孝治
  • Organizer: 第77回応用物理学会秋期学術講演会
  • Place of Presentation: 新潟（朱鷺メッセ）
  • Year and Date: 2016-09-13
• [Presentation] 多重周波数フォースモジュレーション法：細胞レオロジーの定量マッピング (2016)
  • Author(s): 岡嶋孝治
  • Organizer: 顕微鏡学会第72回学術講演会
  • Place of Presentation: 仙台（仙台国際センター）
  • Year and Date: 2016-06-14
  • Invited
• [Presentation] Multi-frequency force modulation atomic force microscopy:Simultaneous measurements of living cells in frequency and time domains (2015)
  • Author(s): R. Takahashi*, T. Okajima
  • Organizer: 23rd International Colloquium on Scanning Probe Microscopy (ICSPM23)
  • Place of Presentation: ニセコ（ヒルトンホテル）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] 原子間力顕微鏡による力学的単一細胞診断：細胞力学量のばらつきの空間依存性 (2015)
  • Author(s): 澤野麻紀*、繁富（栗林）香織、朱きん峰、高橋亮輔、スバギョ・アグス、末岡和久、岡嶋孝治
  • Organizer: 第53回生物物理学会
  • Place of Presentation: 金沢（金沢大）
  • Year and Date: 2015-09-13
• [Presentation] 原子間力顕微鏡：細胞力学特性の個性を測る (2015)
  • Author(s): 岡嶋孝治
  • Organizer: 第53回生物物理学会
  • Place of Presentation: 金沢（金沢大）
  • Year and Date: 2015-09-13
  • Invited
• [Book] ケミカルエンジニヤリング (2015)
  • Author(s): 高橋亮輔、岡嶋孝治
  • Total Pages: 6
  • Publisher: 化学工業社
• [Patent(Industrial Property Rights)] 原子間力顕微鏡による単一細胞の力学的診断方法と診断装置 (2017)
  • Inventor(s): 岡嶋孝治、高橋亮輔
  • Industrial Property Rights Holder: 岡嶋孝治、高橋亮輔
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2017-055414
  • Filing Date: 2017-03-22