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Biomembrane as quasi-nonequilibrium field: molecular diffusion on a membrane and Selective molecular aggregation

Research Project

Project/Area Number 15K13546
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biological physics/Chemical physics/Soft matter physics
Research InstitutionUniversity of Tsukuba

Principal Investigator

HISHIDA Mafumi  筑波大学, 数理物質系, 助教 (70519058)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords水和 / 電気二重層斥力 / 膜間距離 / リン脂質 / Gibbs-Donnan平衡 / 拡散 / リン脂質膜 / リン脂質二重膜 / 水和水
Outline of Final Research Achievements

To investigate molecular diffusion in aqueous phase between bilayers, we tried to establish the method for controlling the lamellar repeat distances. Although controls by humidity change or by using a neutrally charged lipid were found to be difficult, we could establish the method by using charged lipid bilayers experimentally and theoretically. Electrostatic double-layer force obtained by considering Gibbs-Donnan equilibrium well agreeed with experiments. This result reverses the classical model of double-layer force, which has been widely believed for long.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (27 results)

All 2018 2017 2016 2015 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (23 results) (of which Int'l Joint Research: 6 results,  Invited: 6 results) Remarks (1 results)

  • [Journal Article] Electrostatic double-layer interaction between stacked charged bilayers2017

    • Author(s)
      Hishida Mafumi, Nomura Yoko, Akiyama Ryo, Yamamura Yasuhisa, Saito Kazuya
    • Journal Title

      PHYSICAL REVIEW E

      Volume: 96 Issue: 4 Pages: 040601-040601

    • DOI

      10.1103/physreve.96.040601

    • NAID

      120007134697

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salt Effects on Lamellar Structure of Nonionic Surfactants2016

    • Author(s)
      Mafumi Hishida, Yohei Kaneko, Yasuhisa Yamamura, Kazuya Saito
    • Journal Title

      J. Solution Chem.

      Volume: 45 Issue: 11 Pages: 1612-1619

    • DOI

      10.1007/s10953-016-0529-z

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Contrasting Effects of a Rigid Core and an Alkyl Chain in nCB on the Phase Behavior of Lipid Bilayers2016

    • Author(s)
      H. Usuda, M. Hishida, Y. Yamamura & K. Saito
    • Journal Title

      Langmuir

      Volume: 32 Issue: 23 Pages: 5966-5972

    • DOI

      10.1021/acs.langmuir.6b00774

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 荷電脂質膜が作る多重層ベシクルの表面電位2018

    • Author(s)
      菱田真史、野村遥子、秋山良、山村泰久、齋藤一弥
    • Organizer
      第73回年次大会(2018年)
    • Related Report
      2017 Annual Research Report
  • [Presentation] テラヘルツ分光法を用いたソフトマターの水和状態の観測 および 水和状態と自己組織化構造との相互関係 に関する研究2018

    • Author(s)
      菱田真史
    • Organizer
      第73回年次大会(2018年)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ゲル-液晶相転移に伴うリン脂質二重膜間の水の状態変化2017

    • Author(s)
      逸見優太,菱田真史,山村泰久,齋藤一弥
    • Organizer
      日本物理学会 第72回年次大会
    • Place of Presentation
      大阪大学(大阪府豊中市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 荷電性リン脂質膜同士に働く 電気二重層斥力 ~co-ionの効果~2017

    • Author(s)
      菱田真史、野村遥子、秋山良、山村泰久、齋藤一弥
    • Organizer
      つくばソフトマター2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 荷電性リン脂質膜間に働く電気二重層相互作用:co-ionの価数依存性から2017

    • Author(s)
      菱田真史、野村遥子、秋山良、山村泰久、齋藤一弥
    • Organizer
      日本物理学会2017年秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 荷電性リン脂質膜同士に働く 電気二重層斥力 ~co-ionの効果~2017

    • Author(s)
      菱田真史、野村遥子、秋山良、山村泰久、齋藤一弥
    • Organizer
      The 11th Mini-Symposium on Liquids
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Changes in the hydration states of phospholipid bilayers accompanying bilayer structural changes: From the studies by THz spectroscopy2017

    • Author(s)
      Mafumi Hishida
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Hydration states of phospholipids studied by THz spectroscopy2017

    • Author(s)
      Mafumi Hishida、 Yuta Hemmi、 Yasuhisa Yamamura、 Kazuya Saito
    • Organizer
      The 10th Liquid Matter Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] テラヘルツ分光によるPEリン脂質のラメラ液晶-逆ヘキサゴナル相転移に伴う水和状態の変化の観測2017

    • Author(s)
      逸見優太、菱田真史、山村泰久、齋藤一弥
    • Organizer
      平成29年度 日本分光学会年次講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲル-液晶相転移に伴うリン脂質二重膜間の水の状態変化2017

    • Author(s)
      逸見優太、菱田真史、山村泰久、齋藤一弥
    • Organizer
      日本物理学会 第72回年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] PEリン脂質のラメラ液晶‐逆ヘキサゴナル相転移に伴う水和状態の変化2016

    • Author(s)
      逸見 優太, 菱田 真史, 山村 泰久, 齋藤 一弥
    • Organizer
      第 39 回溶液化学シンポジウム
    • Place of Presentation
      産業技術総合研究所(茨城県つくば市)
    • Year and Date
      2016-11-09
    • Related Report
      2016 Research-status Report
  • [Presentation] リン脂質二重膜の物性に対する 添加分子の効果 ~統一的理解に向けて~2016

    • Author(s)
      菱田真史
    • Organizer
      第52回熱測定討論会
    • Place of Presentation
      徳島大学(徳島県徳島市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] アニオン性リン脂質膜の凝集挙動における DLVO理論との不一致2016

    • Author(s)
      菱田真史、野村遥子、山村泰久、齋藤一弥
    • Organizer
      第67回コロイドおよび界面化学討論会
    • Place of Presentation
      北海道教育大学旭川校(北海道旭川市)
    • Year and Date
      2016-09-21
    • Related Report
      2016 Research-status Report
  • [Presentation] テラヘルツ分光によるPEリン脂質のラメラ液晶‐逆ヘキサゴナル相転移に伴う水和状態の変化の観測2016

    • Author(s)
      逸見 優太, 菱田 真史, 山村 泰久, 齋藤 一弥
    • Organizer
      第67回コロイドおよび界面化学討論会
    • Place of Presentation
      北海道教育大学旭川校(北海道旭川市)
    • Year and Date
      2016-09-21
    • Related Report
      2016 Research-status Report
  • [Presentation] A Rigid Core Part and an Alkyl Chain in nCB Bring Contrasting Effects on the Phase Behavior of Lipid Bilayers2016

    • Author(s)
      H. Usuda, M. Hishida, Y. Yamamura, K. Saito
    • Organizer
      71st Calorimery Conference
    • Place of Presentation
      Turtle Bay Resort, Oahu, Hawaii, USA
    • Year and Date
      2016-07-31
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] "Toward systematic understanding of effects of incorporated molecules on phospholipid bilayers "2016

    • Author(s)
      Mafumi Hishida
    • Organizer
      Joint Meeting of The Japan Society of Calorimetry and Thermal Analysis and The Calorimetry Conference
    • Place of Presentation
      Turtle Bay Resort, Oahu, Hawaii, USA
    • Year and Date
      2016-07-31
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 脂質膜の相挙動に対する液晶分子nCBの添加効果2016

    • Author(s)
      臼田初穂,菱田真史,山村泰久,齋藤一弥
    • Organizer
      第15回関東ソフトマター研究会
    • Place of Presentation
      首都大学東京(東京都八王子市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Enhanced order of lipid bilayer by n-alkane2015

    • Author(s)
      Mafumi Hishida, Asami Endo, Ryuta Yanagisawa, Koyomi Nakazawa, YasuhisYamamura, Kazuya Saito
    • Organizer
      Pacifichem2015( 2015 International Chemical Congress of Pacific Basin Societies)
    • Place of Presentation
      Hawaii Convention Center(ホノルル、アメリカ)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] アルカンを含む三成分リン脂質膜の相分離挙動2015

    • Author(s)
      菱田真史,柳澤隆大、山村泰久、齋藤一弥
    • Organizer
      日本物理学会2015年秋季大会
    • Place of Presentation
      関西大学(大阪府吹田市)
    • Year and Date
      2015-09-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] イオンにより誘起される非イオン性界面活性剤表面での水の配向 ~ホフマイスター効果のメカニズムの理解に向けて~2015

    • Author(s)
      菱田真史、金子陽平、奥野将成、山村泰久、 石橋孝章、齋藤一弥
    • Organizer
      第66回コロイドおよび界面化学討論会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Research-status Report
  • [Presentation] n-アルカン添加によるリン脂質二重膜の硬化とベシクルの破裂2015

    • Author(s)
      菱田真史、柳澤隆大、遠藤麻未、山村泰久、 齋藤一弥
    • Organizer
      第66回コロイドおよび界面化学討論会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Research-status Report
  • [Presentation] DPPC 二重膜の相転移挙動に対する 液晶分子nCB の添加効果2015

    • Author(s)
      臼田初穂、菱田真史、長友重紀、山村泰久、齋藤一弥
    • Organizer
      第66回コロイドおよび界面化学討論会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Research-status Report
  • [Presentation] Lamellar repeat distances of charged phospholipid membranes dependent on charges of added co-ions2015

    • Author(s)
      野村遥子、菱田真史、山村泰久、長友重紀、齋藤一弥
    • Organizer
      The 9th Mini-Symposium on Liquids
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2015-07-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 菱田真史ホームページ

    • URL

      https://sites.google.com/site/mafumihishida/

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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