| Project/Area Number |
15K13548
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological physics/Chemical physics/Soft matter physics
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Minoda Hiroki 東京農工大学, 工学(系)研究科(研究院), 教授 (20240757)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | カソードルミネッセンス / 走査電子顕微鏡 / 発光 / GFP / 電子顕微鏡 / 蛍光 / 蛍光タンパク質 / 電子光子相関顕微鏡法 / 発光分光 / イメージング |
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| Outline of Final Research Achievements |
In the present project, the possibility of super high resolution optical microscopy is investigated. Focused electron beam is illuminated onto the enhanced green fluorescent proteins (EGFP) and the electron induced light emission (cathodoluminescence; CL) is detected to form "optical microscope". The factors that affects the stability of the light emission from the EGFP were investigated. The stability does not depend on Ph of the sample solution. When glycerol is included in addition to the EGFP, the EGFP keeps to emit light, however, it is quenching without the glycerol. This clearly shows that the glycerol has an important role to stabilize the GFP.
An optical system to create CL images was constructed and the CL images of the fluorescent diamond could be obtained. However, the detection efficiency of the light is not high enough and more efficient light detector is necessary.
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