Challenge to measure IR spectrum of fluorescent protein chromophore
| Project/Area Number |
15K13621
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Okayama University of Science (2016-2017)
Tokyo Institute of Technology (2015) |
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Principal Investigator |
Sakai Makoto 岡山理科大学, 理学部, 教授 (60298172)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 赤外分光 / タンパク質 / 発色団 / 超解像 / フラビン / 赤外顕微分光 / ローダミン６G |
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| Outline of Final Research Achievements |
IR spectroscopy is one of the powerful tools for investigation of structure of proteins. However, it is difficult to measure IR spectra in aqueous aqueous solution because large IR absorption of water. In this study, we developed a novel transient fluorescence-detected resonance IR spectroscopy, and succeeded the measurement of IR spectrum of flavin mononucleotide, that is one of the fluorescent protein chromosphere, in aqueous solution. Measured spectrum was compared with these measured by Raman spectroscopy. These results will be regarded as the data very useful for the measurement of IR spectrum of fluorescence protein chromosphere.
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Report
(4 results)
Research Products
(21 results)
