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Challenge to measure IR spectrum of fluorescent protein chromophore

Research Project

Project/Area Number 15K13621
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Physical chemistry
Research InstitutionOkayama University of Science (2016-2017)
Tokyo Institute of Technology (2015)

Principal Investigator

Sakai Makoto  岡山理科大学, 理学部, 教授 (60298172)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords赤外分光 / タンパク質 / 発色団 / 超解像 / フラビン / 赤外顕微分光 / ローダミン6G
Outline of Final Research Achievements

IR spectroscopy is one of the powerful tools for investigation of structure of proteins. However, it is difficult to measure IR spectra in aqueous aqueous solution because large IR absorption of water. In this study, we developed a novel transient fluorescence-detected resonance IR spectroscopy, and succeeded the measurement of IR spectrum of flavin mononucleotide, that is one of the fluorescent protein chromosphere, in aqueous solution. Measured spectrum was compared with these measured by Raman spectroscopy. These results will be regarded as the data very useful for the measurement of IR spectrum of fluorescence protein chromosphere.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 2015 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Remarks (3 results)

  • [Journal Article] Mass Analyzed Threshold Ionization Detected Infrared Spectroscopy: Isomerization Activity of the Phenol–Ar Cluster near the Ionization Threshold2015

    • Author(s)
      Mitsuhiko Miyazaki, Shunpei Yoshikawa, François Michels, Kentaro Misawa, Shun-ichi Ishiuchi, Makoto Sakai, Otto Dopfer, Klaus Müller-Dethlefs, Masaaki Fujii
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 17 Issue: 4 Pages: 2949-2503

    • DOI

      10.1039/c4cp04584j

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 赤外超解像顕微鏡法2015

    • Author(s)
      酒井誠,藤井正明
    • Journal Title

      高分子

      Volume: 64 Pages: 588-589

    • Related Report
      2015 Research-status Report
    • Acknowledgement Compliant
  • [Journal Article] 赤外顕微鏡によるがん細胞の生体反応計測技術2015

    • Author(s)
      酒井誠
    • Journal Title

      光学

      Volume: 38 Pages: 344-349

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 超解像赤外分光イメージングによる生体試料観察2017

    • Author(s)
      酒井誠
    • Organizer
      日本分光学会中国四国支部平成28年度年次講演会「画像観測による,分子~生体~凝集系研究の新展開」
    • Place of Presentation
      広島大学(東広島)
    • Year and Date
      2017-01-10
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 赤外超解像顕微鏡を用いた生体試料中におけるケラチンタンパク質の分布・配向観察2017

    • Author(s)
      岩﨑雄一朗、木村裕也、山本幸汰、森本遼、高橋広奈、酒井誠
    • Organizer
      平成29年度日本分光学会年次講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] VSFG検出赤外超解像顕微鏡法による羽毛β-ケラチンの赤外分光イメージング-偏光依存性測定による分子配向、キラリティーの観測-2017

    • Author(s)
      高橋広奈、山本幸汰、木村裕也、渡瀬五常、藤井正明、酒井誠
    • Organizer
      第11回分子科学討論会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Orientation-sensitive molecular imaging of feather β-keratins by a VSFG-detected IR super-resolution microscopy2017

    • Author(s)
      Kota Yamamoto, Yuya Kimura, Kosuke Tatekabe, Tomoya Miyake, Hirona Takahashi, Makoto Sakai
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Molecular distribution and orientation of keratins in human nails and human hairs observed by VSFG-detected IR super resolution microscopy2017

    • Author(s)
      Y. Iwasaki, M . Hata , M. Fujiwara, R. Morimoto, H. Takahashi, M. Sakai
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 生体・環境分子を観察するための赤外超解像顕微鏡の開発2017

    • Author(s)
      酒井誠
    • Organizer
      第54回フローインジェクション分析講演会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 赤外超解像顕微鏡の開発とその応用2016

    • Author(s)
      酒井誠
    • Organizer
      高分子表面研究会「最先端の顕微鏡/イメージング技術で、表面・界面を見る」
    • Place of Presentation
      東京理科大学(東京)
    • Year and Date
      2016-10-21
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Orientation-sensitive imaging of feather β-keratins by VSFG-detected IR super-resolution microscopy2016

    • Author(s)
      Yukihisa Watase, Masaaki Fujii, Makoto Sakai
    • Organizer
      WRHI International Workshop on Advanced Laser Spectroscopy for Soft Molecular Systems
    • Place of Presentation
      Tokyo Institute of Technology (Yokohama)
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] VSFG検出赤外超解像顕微鏡法による羽毛β-ケラチンの分子配向イメージング2016

    • Author(s)
      渡瀬五常,藤井正明,酒井誠
    • Organizer
      第10回分子科学討論会
    • Place of Presentation
      神戸ファッションマート(神戸)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
  • [Presentation] 赤外超解像顕微鏡による生体試料観察2016

    • Author(s)
      酒井誠
    • Organizer
      レーザ顕微鏡研究会第42回講演会
    • Place of Presentation
      理化学研究所(和光)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Orientation-sensitive IR imaging of feather β-keratins by a VSFG-detected IR super-resolution microscopy2015

    • Author(s)
      Yukihisa Watase, Kohei Ushio, Masaaki Fujii, Makoto Sakai
    • Organizer
      International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hawaii Convention Center、Honolulu
    • Year and Date
      2015-12-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Observation of molecular orientation of human hair α-keratin fibers by a VSFG detected IR super-resolution microscopy2015

    • Author(s)
      Makoto Sakai, Kohei Ushio, Yukihisa Watase, Masaaki Fujii
    • Organizer
      International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hawaii Convention Center、Honolulu
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 赤外超解像顕微鏡法による羽毛β-ケラチンの分子配向イメージング2015

    • Author(s)
      渡瀬五常、牛尾公平、藤井正明、酒井誠
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京工業大学 大岡山キャンパス
    • Year and Date
      2015-09-19
    • Related Report
      2015 Research-status Report
  • [Presentation] VSFG検出赤外超解像顕微鏡法を用いた羽毛β-ケラチンの分子配向イメージング2015

    • Author(s)
      渡瀬五常、牛尾公平、藤井正明、酒井誠
    • Organizer
      第53回生物物理学会年会
    • Place of Presentation
      金沢大学角間キャンパス
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
  • [Presentation] VSFG検出赤外超解像顕微鏡法を用いた羽毛β-ケラチンの赤外分光イメージング~Amide I バンドの偏光依存性測定~2015

    • Author(s)
      渡瀬五常、牛尾公平、藤井正明、酒井誠
    • Organizer
      平成27年度日本分光学会年次講演会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Year and Date
      2015-06-01
    • Related Report
      2015 Research-status Report
  • [Remarks] 教員・研究室 - 岡山理科大学 理学部 化学科

    • URL

      http://www.chem.ous.ac.jp/teacher/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 岡山理科大学理学部化学科教員・研究室

    • URL

      http://www.chem.ous.ac.jp/teacher/

    • Related Report
      2016 Research-status Report
  • [Remarks] 藤井・酒井研究室 東京工業大学 資源化学研究所 分光化学部門

    • URL

      http://www.csd.res.titech.ac.jp/indexj.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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