| Project/Area Number |
15K13667
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional solid state chemistry
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| Research Institution | University of Toyama |
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Principal Investigator |
Inouye Masahiko 富山大学, 大学院医学薬学研究部(薬学), 教授 (60211752)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIMOTO Kazuhisa 九州産業大学, 工学部, 准教授 (40334718)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ロタキサン / 蛍光色素 / 高光耐久性 / バイオプローブ / 光安定性 / シクロデキストリン / 一重項酸素 |
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| Outline of Final Research Achievements |
We have developed [3]rotaxane-type fluorophores, in which an alkynylpyrene is encapsulated with two permethyl α -cyclodextrins (PM α-CD). Conclusive proof for the [3]rotaxane structure was obtained on the basis of its X-ray structure analysis. This fluorophore showed extremely high photo-stability because PM α-CD, like bulletproof glass, protected the alkynylpyrene core from the attack of reactive species. Fluorescence spectrum measurement revealed that the [3]rotaxane-type alkynylpyrene emitted only blue light of monomeric pyrene in the various circumstances such as lipophilic, hydrophilic, and even condensed states.
Aiming at evolution of the rotaxane-type fluorophore to biomolecule-labeling agents, we synthesized new [3]rotaxane-type alkynylpyrens with succinimidyl ester. After labeling the protein with this rotaxane, photo-bleaching experiment was executed. It was found that the extremely high photostability of the rotaxane is maintained even after labeling the protein.
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